Mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis
Mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis
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DOI：
10.3748/wjg.v18.i14.1628
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发表时间：
2012-04-14
影响因子：
4.3
通讯作者：
Olsson, Crister
中科院分区：
文献类型：
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作者：
Gustafsson, Rita J.;Ohlsson, Bodil;Olsson, Crister
目的：采用非培养的方法对2例经组织学确诊为胶原性结肠炎的女性患者的结肠微生物区系进行研究。用通用细菌引物扩增出细菌165个rRNA基因。然后将这些扩增片段克隆到感受态大肠杆菌细胞中。结果：这些克隆可分为44个不同的系统发育类型。微生物区系以拟杆菌属和拟杆菌属为主。在两例患者中均发现7个系统型，占克隆总数的47.5%。其中，最主要的是类杆菌中类似于纤维硅拟杆菌、蝉类杆菌、塞陶米隆类杆菌、均匀类杆菌和多雷类杆菌的克隆。两例患者还发现了与普氏杆菌和香茅梭菌相似的序列。结论：发现了潜在致病类杆菌的优势种，并发现了与梭状芽孢杆菌相似的克隆，但整个结肠微生物区系与健康菌相似。胶原性结肠炎的病因也必须被考虑，而不是有害的细菌菌群。(C)2012年白石登。版权所有。
AIM: To characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.METHODS: Biopsies were taken from the ascending colon and the total DNA was extracted. Universal bacterial primers were used to amplify the bacterial 165 rRNA genes. The amplicons were then cloned into competent Escherichia coli cells. The clones were sequenced and identified by comparison to known sequences.RESULTS: The clones could be divided into 44 different phylotypes. The microbiota was dominated by Firmicutes and Bacteroidetes. Seven phylotypes were found in both patients and constituted 47.5% of the total number of clones. Of these, the most dominating were clones similar to Bacteroides cellulosilyticus, Bacteroides caccae, Bacteroides thetaiotaomicron, Bacteroides uniformis and Bacteroides dorei within Bacteroidetes. Sequences similar to Faecalibacterium prausnitzii and Clostridium citroniae were also found in both patients.CONCLUSION: A predominance of potentially pathogenic Bacteroides spp., and the presence of clones showing similarity to Clostridium clostridioforme were found but the overall colon microbiota showed similarities to a healthy one. Etiologies for collagenous colitis other than an adverse bacterial flora must also be considered. (C) 2012 Baishideng. All rights reserved.