Amplified-fragment length polymorphism analysis of Propionibacterium isolates implicated in contamination of blood products
Amplified-fragment length polymorphism analysis of Propionibacterium isolates implicated in contamination of blood products
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DOI：
10.1111/j.1365-2141.2005.05771.x
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发表时间：
2005-11-01
影响因子：
6.5
通讯作者：
Savelkoul, PHM
中科院分区：
文献类型：
--
作者：
Mohammadi, T;Reesink, HW;Savelkoul, PHM
痤疮丙酸杆菌与大多数血小板浓缩物（PC）细菌污染病例有关。为了确定污染源，应用扩增片段长度多态性（AFLP）分析。该DNA指纹技术被用来研究44个分离物的分子关系，来自22个PC和22个相应的红细胞浓缩物（RBC）从相同的全血捐赠。AFLP结果与16 S核糖体RNA基因的1200 bp的测序分析一起揭示了三个主要组的存在：两组（组2和3）（55%）由不源自皮肤植物群的分离物组成，另一组（组1）（45%）由属于皮肤植物群的细菌组成。后一组显示与痤疮丙酸杆菌的参考菌株完全同源。因此，这些分离株可被认为是痤疮丙酸杆菌菌株。相比之下，来自组2和组3的污染物显示与皮肤表面上发现的痤疮丙酸杆菌在分子上无关。AFLP是可重复的，并提供了宝贵的信息的性质丙酸杆菌污染的个人电脑。为了更深入地了解污染源，可以在进一步的研究中利用这种技术来确定血液制品中常见的不同细菌的分子关系。
Propionibacterium acnes is implicated in most cases of bacterial contamination of platelet concentrates (PCs). To determine the source of contamination, amplified-fragment length polymorphism (AFLP) analysis was applied. This DNA fingerprinting technique was used to study the molecular relationship of 44 isolates derived from 22 PCs and 22 corresponding red blood cells concentrates (RBCs) from the same whole blood donations. The AFLP results together with sequencing analysis of the 1200 bp of the 16S ribosomal RNA gene revealed the existence of three main groups: two groups (groups 2 and 3) (55%) consisted of isolates that did not originate from skin flora and another group (group 1) (45%) comprised bacteria belonging to the skin flora. This latter group showed complete homology with reference strains of P. acnes. Therefore these isolates can be considered as P. acnes strains. In contrast, contaminants from groups 2 and 3 were shown to be molecularly unrelated to the P. acnes found on the skin surface. The AFLP is reproducible and gave invaluable information about the nature of Propionibacteria contaminating PCs. To gain more insights into the source of contamination, this technique could be exploited in further studies to determine the molecular relationship of different bacteria commonly found in blood products.