Multi-parameter measurements of conformational dynamics in nucleic acids and nucleoprotein complexes.
Multi-parameter measurements of conformational dynamics in nucleic acids and nucleoprotein complexes.
复制标题
核酸和核蛋白复合物构象动力学的多参数测量。
DOI：
10.1016/j.ymeth.2019.06.019
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Bryant,Zev
中科院分区：
文献类型：
--
作者：
Ivanov,IvanE;Bryant,Zev
生物大分子经历动态构象变化。单分子方法可以实时跟踪这种结构重排。然而，虽然大分子的结构可能会沿着许多自由度变化，但单分子技术只能监测有限数量的这些运动轴。正在开发先进的单分子方法，以高分辨率跟踪核酸和核蛋白复合体中的多个自由度，以便能够更好地操纵和控制正在研究的系统，并以大规模并行的方式收集测量结果。在同一化验中结合互补的单分子方法也提供了独特的测量机会。结合荧光和FRET的磁性和光学镊子的实施已经证明了仅靠这两种技术都不能达到的结果。将其他先进的单分子方法与荧光检测相结合，将使我们能够更好地捕捉生物学中心的核酸和核蛋白复合体的多维动力学。
Biological macromolecules undergo dynamic conformational changes. Single-molecule methods can track such structural rearrangements in real time. However, while the structure of large macromolecules may change along many degrees of freedom, single-molecule techniques only monitor a limited number of these axes of motion. Advanced single-molecule methods are being developed to track multiple degrees of freedom in nucleic acids and nucleoprotein complexes at high resolution, to enable better manipulation and control of the system under investigation, and to collect measurements in massively parallel fashion. Combining complementary single-molecule methods within the same assay also provides unique measurement opportunities. Implementations of magnetic and optical tweezers combined with fluorescence and FRET have demonstrated results unattainable by either technique alone. Augmenting other advanced single-molecule methods with fluorescence detection will allow us to better capture the multidimensional dynamics of nucleic acids and nucleoprotein complexes central to biology.