Dually functionalized dendrimers by temperature-sensitive surface modification and gold nanoparticle loading for biomedical application
Dually functionalized dendrimers by temperature-sensitive surface modification and gold nanoparticle loading for biomedical application
复制标题
DOI：
10.1039/c4ra03947e
复制
发表时间：
2014-06
期刊：
影响因子：
3.9
通讯作者：
K. Kono;K. Takeda;Xiao-jie Li;E. Yuba;Atsushi Harada;T. Ozaki;S. Mori
中科院分区：
文献类型：
--
作者：
K. Kono;K. Takeda;Xiao-jie Li;E. Yuba;Atsushi Harada;T. Ozaki;S. Mori
通过使用丙氧基二甘醇（PDEG）和金纳米颗粒（AuNP）负载对聚酰胺胺（PAMAM）树枝状聚合物进行温度敏感表面修饰的双重功能化，开发了一种新型刺激敏感树枝状聚合物。这些 AuNP 杂化树枝状聚合物是通过使用 4-硝基苯基氯甲酸酯将 PDEG 连接到胺封端的 PAMAM G5 树枝状聚合物上，然后通过在树枝状聚合物内部用 NaBH4 还原 AuCl4− 离子将 AuNP 负载到树枝状聚合物内部来制备的。透射电子显微镜显示，直径约2 nm的AuNPs保留在PDEG修饰的树枝状聚合物中。这些负载 AuNP 的树枝状聚合物在低温下溶解在水溶液中，但在高于特定温度时变得不溶于水，因为树枝状聚合物表面的特性随温度从亲水性变为疏水性。树枝状聚合物内部的金纳米粒子在绿色激光照射下产生热量，因此，它们的缔合可以在绿色激光照射的特定区域中诱导。此外，温度升高和光照射的结合被证明可以控制它们与细胞的相互作用，从而使 AuNP 杂交的温度敏感树枝状聚合物能够选择性杀死细胞。
Stimuli-sensitive dendrimers of a new type were developed through dual functionalization of polyamidoamine (PAMAM) dendrimers with temperature-sensitive surface modification using propoxy diethylene glycol (PDEG) and Au nanoparticle (AuNP) loading. These AuNP-hybridized dendrimers were prepared by attaching PDEG to amine-terminated PAMAM G5 dendrimer using 4-nitrophenylchloroformate and subsequent loading of AuNPs into the dendrimer interior via reduction of AuCl4− ions with NaBH4 in the dendrimer interior. Transmission electron microscopy showed that AuNPs with diameters of about 2 nm were retained in the PDEG-modified dendrimers. These AuNP-loaded dendrimers were dissolved in aqueous solutions at low temperature but became water-insoluble above a specific temperature, because of the character change of the dendrimer surface from hydrophilic to hydrophobic depending on temperature. The AuNPs in the dendrimer interior generated heat under irradiation by a green laser and hence, their association could be induced in the specific area irradiated with the green laser. In addition, the combination of the temperature increase and light irradiation was shown to control their interaction with cells, enabling selective cell killing by the AuNP-hybridized temperature-sensitive dendrimers.