A modified, sensitive liquid chromatographic method for measurement of propranolol with fluorescence detection.
A modified, sensitive liquid chromatographic method for measurement of propranolol with fluorescence detection.
复制标题
一种改进的、灵敏的液相色谱方法，用于通过荧光检测测量普萘洛尔。
DOI：
10.1002/jps.2600750121
复制
发表时间：
1986
影响因子：
3.8
通讯作者：
Wood,AJ
中科院分区：
文献类型：
--
作者：
Koshakji,RP;Wood,AJ
建立了一种简便、灵敏、快速的人和动物血浆及血液中普萘洛尔浓度的荧光高效液相色谱测定方法。该方法需要流动相混合物中的微升三乙胺和微升的血浆或血液(50-100μL)，可用于常规临床分析以及使用小型实验动物进行的药代动力学研究。在分析之前，一个单一的、选择性的提取步骤。最低检测浓度为1 ng/m L。
A simple, sensitive, and rapid fluorometric‐high‐perform‐ance liquid Chromatographic method for quantitating human and animal plasma and blood levels of propranolol has been developed. The procedure, which requires microliter volumes of triethylamine in the mobile phase mixture and microliter volumes of plasma or blood (50–100 μL), may be applied in routine clinical analysis as well as in pharmacokinetic studies using small laboratory animals. A single, selective extraction step precedes the analysis. The limiting concentration that can be detected is 1 ng/mL.