Monoclonal antibodies that detect biochemical markers of arthritis in humans.
Monoclonal antibodies that detect biochemical markers of arthritis in humans.
复制标题
DOI：
10.1002/art.1780380513
复制
发表时间：
1995-05
影响因子：
--
通讯作者：
R. R. Slater-R.;M. Bayliss;P. Lachiewicz;D. Visco;B. Caterson
中科院分区：
文献类型：
--
作者：
R. R. Slater-R.;M. Bayliss;P. Lachiewicz;D. Visco;B. Caterson
目的探讨用单抗3-B-3(-)和7-D-4检测关节软骨代谢改变的生化标志物的可能性。方法收集55例正常关节软骨标本(年龄18周~83岁)和89例关节炎软骨标本(年龄20~81岁)，提取其蛋白多糖，分析其是否存在单抗3-B-3和7-D-4识别的表位。结果骨关节炎、幼年类风湿性关节炎、类风湿性关节炎、缺血性坏死、退行性半月板撕裂等关节炎患者软骨提取的蛋白多糖中，Native 3-B-3(-)模拟表位的表达频率较高。它在正常软骨标本中的表达很低或不表达，主要发生在年轻、骨骼不成熟的个体中。相反，7-D-4表位在不同关节炎患者中的表达差异较大，在正常软骨标本中也经常发现。免疫组织化学3-B-3(-)和7-D-4免疫组织化学分析显示，软骨退变、重塑和修复最严重的浅表区域有局灶性阳性染色。结论单抗3-B-3(-)和7-D-4识别的生化标志物反映了人关节软骨蛋白多糖合成和代谢的改变。这些数据表明，在人类软骨中，3-B-3(-)表位可能比7-D-4表位更能反映生物化学变化。
OBJECTIVE To evaluate the potential of using monoclonal antibodies (MAb) 3-B-3(-) and 7-D-4 to detect biochemical markers of altered cartilage metabolism in human arthritides. METHODS Fifty-five samples of normal articular cartilage (subjects' age range 18 weeks of gestation to 83 years of age) and 89 samples of arthritic cartilage (patients' age range 20-81 years) were collected, and their proteoglycans were extracted and analyzed for the presence of the epitopes recognized by MAb 3-B-3 and 7-D-4. RESULTS Native 3-B-3(-) mimotope was expressed at a high incidence in proteoglycans extracted from the cartilage of patients with most of the arthritic diseases examined (osteoarthritis, juvenile rheumatoid arthritis, rheumatoid arthritis, avascular necrosis, and degenerative meniscal tears). Its expression in normal cartilage specimens was very low or absent, occurring mainly in the young, skeletally immature individuals. In contrast, expression of the 7-D-4 epitope was more variable in patients with different arthritides and was also frequently found in normal cartilage specimens. Immunohistochemical analyses with both 3-B-3(-) and 7-D-4 showed strong focal positive staining in superficial areas, where cartilage degeneration, remodeling, and repair were greatest. CONCLUSION The biochemical markers recognized by MAb 3-B-3(-) and 7-D-4 are indicative of altered proteoglycan synthesis and metabolism in human articular cartilage. The data suggest that in human cartilage, the 3-B-3(-) epitope might be a better marker of biochemical changes than the 7-D-4 epitope.