Free zinc ions outside a narrow concentration range are toxic to a variety of cells in vitro.
Free zinc ions outside a narrow concentration range are toxic to a variety of cells in vitro.
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DOI：
10.1258/ebm.2010.009258
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发表时间：
2010-06
期刊：
影响因子：
--
通讯作者：
Thompson RB
中科院分区：
文献类型：
--
作者：
Bozym RA;Chimienti F;Giblin LJ;Gross GW;Korichneva I;Li Y;Libert S;Maret W;Parviz M;Frederickson CJ;Thompson RB
锌离子最近在大脑、视网膜、小肠、前列腺、心脏、胰腺和免疫系统等不同部位参与了许多新的功能和病理变化。锌离子是必需的营养物质，但在体外，高浓度的锌离子会杀死细胞。关于锌的影响的自相矛盾的观察经常出现在文献中，而且通常它们的生理学相关性不清楚。我们发现，对于PC-12、HeLa和HT-29细胞以及在不同介质中原代培养的心肌细胞和神经元，大约5nmoL/L游离锌(PZN=8.3PZN定义为−log10[游离锌离子])产生明显健康的细胞，但浓度高20倍或(在一种情况下)低浓度通常是有害的。这些结果表明：(1)培养基中的游离锌离子水平应通过金属离子缓冲来控制；(2)在缓冲不足的介质中添加锌或强锌配体可能会导致无法预测的游离锌水平，这往往对细胞有害；以及(3)由于许多生物化学物质中存在污染锌，并且许多介质的缓冲能力未知，因此通常需要测量游离锌离子水平。
The zinc(II) ion has recently been implicated in a number of novel functions and pathologies in loci as diverse as the brain, retina, small intestine, prostate, heart, pancreas and immune system. Zinc ions are a required nutrient but elevated concentrations are known to kill cells in vitro. Paradoxical observations regarding zinc’s effects have appeared frequently in the literature, and often their physiological relevance is unclear. We found that for PC-12, HeLa and HT-29 cell lines as well as primary cultures of cardiac myocytes and neurons in vitro in differing media, approximately 5 nmol/L free zinc (pZn = 8.3, where pZn is defined as − log10 [free Zn2+]) produced apparently healthy cells, but 20-fold higher or (in one case) lower concentrations were usually harmful as judged by multiple criteria. These results indicate that (1) the free zinc ion levels of media should be controlled with a metal ion buffer; (2) adding zinc or strong zinc ligands to an insufficiently buffered medium may lead to unpredictably low or high free zinc levels that are often harmful to cells; and (3) it is generally desirable to measure free zinc ion levels due to the presence of contaminating zinc in many biochemicals and unknown buffering capacity of many media.
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影响因子：
3.9
作者：
Franklin, RB;Ma, J;Costello, LC
通讯作者：
Costello, LC
影响因子：
4.8
作者：
Jiang, DM;Sullivan, PG;Weiss, JH
通讯作者：
Weiss, JH
DOI：
10.1073/pnas.0709307104
发表时间：
2007-11-20
影响因子：
11.1
作者：
Li, Min;Zhang, Yuqing;Yao, Qizhi
通讯作者：
Yao, Qizhi
影响因子：
56.9
作者：
BUSH, AI;PETTINGELL, WH;TANZI, RE
通讯作者：
TANZI, RE
影响因子：
4
作者：
Bozym, Rebecca A.;Thompson, Richard B.;Fierke, Carol A.
通讯作者：
Fierke, Carol A.