Surveillance for avirulent Newcastle disease viruses in domestic ducks (Anas platyrhynchos and Cairina moschata) at live bird markets in Eastern China and characterization of the viruses isolated
Surveillance for avirulent Newcastle disease viruses in domestic ducks (Anas platyrhynchos and Cairina moschata) at live bird markets in Eastern China and characterization of the viruses isolated
复制标题
中国东部活禽市场家鸭（绿头鸭和凯里纳）无毒力新城疫病毒监测及分离病毒特征
DOI：
10.1080/03079450903183637
复制
发表时间：
2009-01-01
期刊：
影响因子：
2.8
通讯作者：
Liu, Xiufan
中科院分区：
文献类型：
--
作者：
Liu, Xiaowen;Wang, Xiaoquan;Liu, Xiufan
在中国东部地区活禽市场为期5年的监测研究中，从家鸭中分离鉴定出新城疫病毒201株。对其中73个菌株进行了生物学和遗传学特征分析。用逆转录-聚合酶链式反应扩增融合蛋白(F)基因，并进行序列测定。1日龄无特定病原体雏鸡的脑内致病指数试验和鸡胚平均死亡时间试验以及F蛋白前体的裂解位点分析表明，它们都是新城疫强毒。基于F基因部分序列的系统发育分析表明，30株毒株聚为I类分支，43株毒株聚为II类基因I，但与中国广泛使用的疫苗病毒昆士兰V4存在差异。大部分I型病毒(18/30)与最近被命名为3型的香港活禽市场毒株形成了一个独立的分支，而其余的I型病毒(12/30)与2型中的一些欧洲病毒关系密切。43株I型I型病毒与最初属于Ia型的病毒全部偏离，并与来自远东的水禽分离株形成了一个单独的亚种Ib，这表明野生水禽和家禽之间可能存在传播。本研究的结果清楚地表明，家鸭群体携带有规律的遗传分化的新城疫强毒，可能是重要的宿主之一。
We isolated and identified 201 Newcastle disease viruses (NDVs) from domestic ducks in a 5-year surveillance study at live bird markets in Eastern China. Seventy-three of these isolates were characterized biologically and genetically. Fusion protein (F) genes of these isolates were amplified by reverse transcription-polymerase chain reaction and sequenced. Intracerebral pathogenicity index tests in 1-day-old specific-pathogen-free chickens and the mean death time of embryonated fowl eggs in addition to the cleavage site analysis of the F-protein precursor for these viruses showed that they were all avirulent NDVs. Phylogenetic analysis based on partial sequences of the F gene showed that 30 isolates clustered into the class I clade and the other 43 isolates clustered into genotype I of class II, but diverged from the vaccine virus Queensland V4, which is extensively used in China. Most class I viruses (18/30) formed a separate branch closest to the Hong Kong live bird market strains that have been recently designated as genotype 3, while the rest (12/30) were closely related to some European viruses within genotype 2. All of the 43 class II genotype I viruses diverged from viruses originally assigned to genotype Ia and formed a separate sublineage designated as Ib with water bird isolates from the Far East, suggesting the possible transmission between the wild and domestic waterfowl. The results in the present study clearly showed that the domestic duck population carries avirulent NDVs with genetic divergence regularly and may act as one of the important reservoirs.