A Role for the EAL-Like Protein STM1344 in Regulation of CsgD Expression and Motility in Salmonella enterica Serovar Typhimurium
A Role for the EAL-Like Protein STM1344 in Regulation of CsgD Expression and Motility in Salmonella enterica Serovar Typhimurium
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DOI：
10.1128/jb.00290-09
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发表时间：
2009-06-15
影响因子：
3.2
通讯作者：
Romling, Ute
中科院分区：
文献类型：
--
作者：
Simm, Roger;Remminghorst, Uwe;Romling, Ute
细菌第二信使环二 GMP (c-di-GMP) 调节静止性和运动性之间的转变。在鼠伤寒沙门氏菌中，多细胞 rdar 形态类型行为的调节因子 CsgD 的表达是 c-di-GMP 信号传导的主要目标。 CsgD 表达受至少两种二鸟苷酸环化酶（GGDEF 结构域蛋白）正向调节，并受至少四种磷酸二酯酶（EAL 结构域蛋白）负向调节。在这里，我们发现，与充当磷酸二酯酶的 EAL 结构域蛋白相反，EAL 样蛋白 STM1344 正向调节 CsgD 表达，负向调节运动。然而，STM1344 在 c-di-GMP 周转中没有作用，也不结合核苷酸。 STM1344 作用于磷酸二酯酶 STM1703 和 STM3611 的上游，此前已发现这两种酶参与 CsgD 下调，并抑制其表达。因此，尽管STM1344在c-di-GMP代谢中没有保留直接作用，但它仍然参与c-di-GMP周转的调节，并在静止性和运动性之间的转换中发挥作用。
The bacterial second messenger cyclic di-GMP (c-di-GMP) regulates the transition between sessility and motility. In Salmonella enterica serovar Typhimurium, the expression of CsgD, the regulator of multicellular rdar morphotype behavior, is a major target of c-di-GMP signaling. CsgD expression is positively regulated by at least two diguanylate cyclases, GGDEF domain proteins, and negatively regulated by at least four phosphodiesterases, EAL domain proteins. Here, we show that in contrast to EAL domain proteins acting as phosphodiesterases, the EAL-like protein STM1344 regulated CsgD expression positively and motility negatively. STM1344, however, did not have a role in c-di-GMP turnover and also did not bind the nucleotide. STM1344 acted upstream of the phosphodiesterases STM1703 and STM3611, previously identified to participate in CsgD downregulation, where it repressed their expression. Consequently, although STM1344 has not retained a direct role in c-di-GMP metabolism, it still participates in the regulation of c-di-GMP turnover and has a role in the transition between sessility and motility.