Totipotent migratory stem cells in a hydroid
Totipotent migratory stem cells in a hydroid
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DOI：
10.1016/j.ydbio.2004.08.006
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发表时间：
2004-11-01
影响因子：
2.7
通讯作者：
Frank, U
中科院分区：
文献类型：
--
作者：
Müller, WA;Teo, R;Frank, U
水母是最古老的真生动物门中的一员，它拥有多潜能的迁移性Stein细胞，寄宿在上皮细胞的间质空间，因此被称为间质细胞或I-细胞。根据传统的理解，基于对Hydra的研究，这些I-细胞可以产生几种细胞类型，如刺细胞、神经细胞和生殖细胞，但不能产生外胚层和内胚层上皮细胞：这些细胞被认为是独立的细胞谱系。我们在这里展示了，在水化细胞中，这些迁移干细胞的发展潜力比之前预期的要广泛。我们从亚克隆野生型动物中剔除了I细胞，随后从突变克隆中引入了I细胞，反之亦然。突变供体和野生型受体在性别、生长方式和形态上存在差异。随着时间的推移，受者完全转变为供者的表型和基因。因此，在这些实验条件下，水生植物的间质干细胞表现出全能性。(C)2004 Elsevier Inc.保留所有权利。
Hydroids, members of the most ancient eumetazoan phylum, the Cnidaria, harbor multipotent, migratory stein cells lodged in interstitial spaces of epithelial cells and are therefore referred to as interstitial cells or i-cells. According to traditional understanding, based on studies in Hydra, these i-cells give rise to several cell types such as stinging cells, nerve cells, and germ cells, but not to ectodermal and endodermal epithelial cells: these are considered to constitute separate cell lineages. We show here that, in Hydractinia, the developmental potential of these migratory stem cells is wider than previously anticipated. We eliminated the i-cells from subcloned wild-type animals and subsequently introduced i-cells from mutant clones and vice versa. The mutant donors and the wild-type recipients differed in their sex, growth pattern, and morphology. With time, the recipient underwent a complete conversion into the phenotype and genotype of the donor. Thus, under these experimental conditions the interstitial stem cells of Hydractinia exhibit totipotency. (C) 2004 Elsevier Inc. All rights reserved.