Differential expression of the pro-inflammatory cytokines IL-1β-1, TNFα-1 and IL-8 in vaccinated pink (Oncorhynchus gorbuscha) and chum (Oncorhynchus keta) salmon juveniles
Differential expression of the pro-inflammatory cytokines IL-1β-1, TNFα-1 and IL-8 in vaccinated pink (Oncorhynchus gorbuscha) and chum (Oncorhynchus keta) salmon juveniles
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DOI：
10.1016/j.fsi.2006.06.012
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发表时间：
2007-04-01
影响因子：
4.7
通讯作者：
Jones, S. R. M.
中科院分区：
文献类型：
--
作者：
Fast, M. D.;Johnson, S. C.;Jones, S. R. M.
实验室饲养的粉红色和小鲑鱼幼鱼(类似于2克)接受了商业的、未加佐剂的沙门氏气单胞菌疫苗或无菌生理盐水的腹膜注射。分别于注射后6h和24 h检测促炎症细胞因子IL-1β1(IL-1β)、肿瘤坏死因子-α(TNFα)和白细胞介素8(IL-8)基因在肾和肝池中的表达水平。IL-1在粉红鲑鱼和鲑鱼中的表达在6h时显著升高，在粉红鲑鱼中的表达在24h时下降，但在chum鲑鱼中的表达不明显。同样，TNFa在两种鱼中的表达在6小时后显著升高，24小时后仅在鲑鱼中显著升高。IL-8的表达在注射后6小时和24小时在两种动物中均显著升高。这三种致炎细胞因子基因的表达在不同的鲑鱼物种中的表达在时间和幅度上都不同。这些差异在这些鱼的免疫功能方面的意义需要进一步研究。皇冠版权所有(C)2006由爱思唯尔有限公司出版。保留所有权利。
Laboratory-reared pink and chum salmon juveniles (similar to 2 g) received an intraperitoneal injection with a commercial, unadjuvanted Aeromonas salmonicida bacterin or sterile saline. Relative to elongation factor-1A, expression levels of genes encoding the proinflammatory cytokines interleukin-1 beta-1 (IL-1 beta), tumour necrosis factor-alpha-1 (TNF alpha) and interleukin-8 (IL-8) in pools of kidney and liver were examined 6- and 24-h after injection. Expression of IL-1 was significantly elevated in pink and chum salmon by 6-h, and declined in pink salmon but not in chum salmon by 24-h. Similarly, expression of TNFa was significantly elevated in both species at 6 h and only in chum salmon after 24-h. Expression of IL-8 was significantly elevated in both species at 6- and 24-h after injection. Expression of the three proinflammatory cytokine genes differed between salmon species both in the timing and magnitude of their expression. The significance of these differences with respect to immune function in these fish requires further research. Crown Copyright (c) 2006 Published by Elsevier Ltd. All rights reserved.