Comprehensive identification and profiling of Chinese shrimp (Fenneropenaeus chinensis) microRNAs in response to high pH stress using Hiseq2000 sequencing
Comprehensive identification and profiling of Chinese shrimp (Fenneropenaeus chinensis) microRNAs in response to high pH stress using Hiseq2000 sequencing
复制标题
使用 Hiseq2000 测序全面鉴定和分析中国明对虾 (Fenneropenaeus chinensis) 响应高 pH 胁迫的 microRNA
DOI：
10.1111/are.14269
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发表时间：
2019
影响因子：
2
通讯作者：
Li Jian
中科院分区：
文献类型：
--
作者：
Li Zhaoxia;Wang Jinye;He Yuying;Hu Shuo;Wang Qingyin;Li Jian
pH值对许多水生动物的分布、生长、行为和生理有很大影响。MicroRNA（miRNAs）是一类小的非编码RNA，在动物对环境应激的反应中起着重要作用。在这项研究中，我们构建了两个小RNA（sRNA）文库，分别来自对照（pH 7.4）和高pH（9.4）处理的虾，以鉴定高pH应激反应的miRNA。使用深度测序，我们从高pH挑战的虾肝胰腺中鉴定了72种（3种保守的和69种新的）上调和4种（2种保守的和2种新的）下调的miRNA。基因本体注释和京都基因百科全书和基因组途径分析揭示，这些靶转录物涉及广泛的生物过程和各种代谢途径。此外，我们通过qRT-PCR验证了所选miRNAs及其靶基因的表达模式。本工作为中国明对虾miRNAs及其潜在靶点的大规模鉴定和表征提供了新的见解，并为在转录后水平上研究动物对高pH胁迫反应的潜在机制奠定了基础。
pH has a great impact on the distribution, growth, behaviour and physiology in many aquatic animals. MicroRNAs (miRNAs), a class of small non‐coding RNAs play essential roles in animal responses to environmental stress. In this study, we constructed two small RNA (sRNA) libraries from control (pH 7.4) and high pH (9.4) treated shrimp to identify high pH stress‐responsive miRNAs. Using deep sequencing, we identified 72 (3 conserved and 69 novel) up‐ and 4 (2 conserved and 2 novel) down‐regulated miRNAs from high pH challenged shrimp hepatopancreas. Gene Ontology annotations and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that these target transcripts were implicated in a broad range of biological processes and various metabolic pathways. Moreover, we validated the expression patterns of selected miRNAs and their target genes by qRT‐PCR. The present work adds new insights into the large‐scale identification and characterization ofFenneropenaeus chinensismiRNAs and their potential targets and represents a foundation for underlying mechanisms of animal responses to high pH stress at post‐transcriptional level.