CHARACTERIZATION OF FULLY 2'-MODIFIED OLIGORIBONUCLEOTIDE HETERODUPLEX AND HOMODUPLEX HYBRIDIZATION AND NUCLEASE SENSITIVITY
CHARACTERIZATION OF FULLY 2'-MODIFIED OLIGORIBONUCLEOTIDE HETERODUPLEX AND HOMODUPLEX HYBRIDIZATION AND NUCLEASE SENSITIVITY
复制标题
DOI：
10.1093/nar/23.11.2019
复制
发表时间：
1995-06-11
影响因子：
14.9
通讯作者：
COOK, PD
中科院分区：
文献类型：
--
作者：
CUMMINS, LL;OWENS, SR;COOK, PD
比较了含有2 ′-氟、2 ′-O-甲基、2 ′-O-丙基和2 ′-O-戊基核苷酸的完全修饰的寡核糖核苷酸序列的核酸酶稳定性和解链温度（T-m）。如通过圆二色谱法所观察到的，在2'修饰的寡核糖核苷酸和RNA之间形成的双链体具有典型的A-型几何形状。观察到修饰（除了2'-O-戊基）增加了与互补RNA形成的双链体的T。修饰的同源双链体显示出显著更高的T（m）s，具有以下T-m顺序：2 '-氟：2'-氟> 2 '-丙基：2'-O-丙基> 2 '-O-甲基：2'-O-甲基> RNA：RNA > DNA：用蛇毒磷酸二酯酶（SVPD）和核酸酶S1检测了2 '-修饰的寡核苷酸的核酸酶稳定性。观察到由2'修饰赋予的稳定性与修饰的大小相关。在具有形成二级结构的潜力的寡核糖核苷酸中存在额外水平的核酸酶稳定性，但仅对于2'修饰的寡核糖核苷酸，而不对于2 '-脱氧寡核糖核苷酸。
The nuclease stability and melting temperatures (T-m) were compared for fully modified oligoribonucleotide sequences containing 2'-fluoro, 2'-O-methyl, 2'-O-propyl and 2'-O-pentyl nucleotides. Duplexes formed between 2' modified oligoribonucleotides and RNA have typical A-form geometry as observed by circular dichroism spectroscopy, Modifications, with the exception of 2'-O-pentyl, were observed to increase the T, of duplexes formed with complementary RNA, Modified homoduplexes showed significantly higher T(m)s, with the following T-m order: 2'-fluoro:2'fluoro > 2'-)-propyl:2'-O-propyl > 2'-O-methyl:2'-O-methyl > RNA:RNA > DNA:DNA, The nuclease stability of 2'-modified oligoribonucleotides was examined using snake venom phosphodiesterase (SVPD) and nuclease S1. The stability imparted by 2' modifications was observed to correlate with the size of the modification, An additional level of nuclease stability was present in oligoribonucleotides having the potential for forming secondary structure, but only for 2' modified oligoribonucleotides and not for 2'-deoxy oligoribonucleotides.