Effect of eucalyptus essential oil on respiratory bacteria and viruses
Effect of eucalyptus essential oil on respiratory bacteria and viruses
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DOI：
10.1007/s00284-007-9045-0
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Quaglio, Paola
中科院分区：
文献类型：
--
作者：
Cermelli, Claudio;Fabio, Anna;Quaglio, Paola
测定了蓝桉精油对120株化脓性链球菌、20株肺炎链球菌、40株无乳链球菌、20株金黄色葡萄球菌、40株流感嗜血杆菌、30株副流感嗜血杆菌、10株肺炎克雷伯菌、10株嗜麦芽寡养单胞菌和两种病毒（一株腺病毒和一株腮腺炎病毒）的活性。均来自呼吸道感染患者的临床标本。采用MTT法对VERO细胞进行细胞毒性评价。采用Kirby Bauer纸片法、最小抑菌浓度和最小杀菌浓度评价其抑菌活性。流感嗜血杆菌、副流感嗜血杆菌和嗜麦芽链球菌最敏感，其次是肺炎嗜血杆菌。通过终点稀释法对腺病毒滴度的病毒产量实验和对腮腺炎病毒的斑块减少试验来评估抗病毒活性，发现对腮腺炎病毒只有轻微的活性。
The activity of Eucalyptus globulus essential oil was determined for 120 isolates of Streptococcus pyogenes, 20 isolates of S. pneumoniae, 40 isolates of S. agalactiae, 20 isolates of Staphylococcus aureus, 40 isolates of Haemophilus influenzae, 30 isolates of H. parainfluenzae, 10 isolates of Klebsiella pneumoniae, 10 isolates of Stenotrophomonas maltophilia and two viruses, a strain of adenovirus and a strain of mumps virus, all obtained from clinical specimens of patients with respiratory tract infections. The cytotoxicity was evaluated on VERO cells by the MTT test. The antibacterial activity was evaluated by the Kirby Bauer paper method, minimum inhibitory concentration, and minimum bactericidal concentration. H. influenzae, parainfluenzae, and S. maltophilia were the most susceptible, followed by S. pneumoniae. The antiviral activity, assessed by means of virus yield experiments titered by the end-point dilution method for adenovirus, and by plaque reduction assay for mumps virus, disclosed only a mild activity on mumps virus.