Specific immunoreactivity of hybridoma-secreted monoclonal anti-melanoma antibodies to cultured cells and freshly derived human cells.
Specific immunoreactivity of hybridoma-secreted monoclonal anti-melanoma antibodies to cultured cells and freshly derived human cells.
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发表时间：
1980-10
期刊：
影响因子：
11.2
通讯作者：
M. Herlyn;W. Clark;M. Mastrangelo;D. Guerry;D. Elder;D. Larossa;R. Hamilton;E. Bondi;R. Tuthill;Z. Steplewski;H. Koprowski
中科院分区：
文献类型：
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作者：
M. Herlyn;W. Clark;M. Mastrangelo;D. Guerry;D. Elder;D. Larossa;R. Hamilton;E. Bondi;R. Tuthill;Z. Steplewski;H. Koprowski
使用放射免疫分析，混合血细胞吸附试验，和各种恶性和非恶性细胞的定量吸收的单克隆抗体对黑色素瘤细胞的特异性进行了分析。六种杂交瘤分泌的抗体中的三种与大多数黑色素瘤细胞系、黑色素瘤肿瘤和星形细胞瘤细胞系以及测试的所有正常和EB病毒转化的淋巴细胞结合。结合模式与人细胞上DR抗原的存在或不存在一致。相反，另外两种抗体19-19和Nu 4 B仅检测到黑素瘤和星形细胞瘤细胞共有的两种不同抗原。黑色素瘤细胞的克隆导致DR阳性和DR阴性克隆的建立，其中Nu 4 B抗体的结合全部保留。
The specificities of monoclonal antibodies against melanoma cells were analyzed using radioimmunoassay, mixed-hemadsorption assay, and quantitative absorption on a variety of malignant and nonmalignant cells. Three of the six hybridoma-secreted antibodies bound to the majority of melanoma cell lines, melanoma tumors, and astrocytoma cell lines as well as to all normal and Epstein-Barr virus-transformed lymphocytes tested. The binding pattern coincides with the presence or absence of the DR antigen on human cells. Conversely, two other antibodies, 19-19 and Nu4B, detected two different antigens common to melanoma and astrocytoma cells only. Cloning of melanoma cells resulted in establishment of DR-positive and DR-negative clones, with the binding of Nu4B antibody retained in all.