Dimensionality-Dependent Mechanical Stretch Regulation of Cell Behavior.
Dimensionality-Dependent Mechanical Stretch Regulation of Cell Behavior.
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DOI：
10.1021/acsami.2c01266
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发表时间：
2022-04
影响因子：
9.5
通讯作者：
Kun Man;Jiafeng Liu;Khang Phan;Kai Wang;Jung Yeon Lee;Xiankai Sun;Michael’s Story;D. Saha;Jun Liao;Hamid Sadat;Yong Yang
中科院分区：
文献类型：
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作者：
Kun Man;Jiafeng Liu;Khang Phan;Kai Wang;Jung Yeon Lee;Xiankai Sun;Michael’s Story;D. Saha;Jun Liao;Hamid Sadat;Yong Yang
各种细胞在体内都会受到机械拉伸，这对细胞、组织和器官的功能和稳态起着关键作用。偏离生理相关的机械拉伸通常与器官功能障碍和各种疾病有关。尽管在一些体外细胞培养模型中提供了机械拉伸，但拉伸维度对细胞的影响常常被忽视，并且拉伸维度是否以及如何影响细胞行为仍不清楚。在这里，我们开发了细胞培养平台，提供1-D单轴，2-D周向或3-D径向机械拉伸，概括了细胞在体内经历的三种主要类型的机械拉伸。我们研究了在这些平台上培养的人微血管内皮细胞和人肺泡上皮细胞的行为，表明机械拉伸以拉伸维度依赖的方式影响细胞形态以及细胞-细胞和细胞-基质相互作用。此外，内皮细胞和上皮细胞分别对生理相关的二维和三维拉伸敏感，这可以促进内皮细胞和上皮细胞的形成。这项研究强调了在体外组织/器官模型的开发中重建生理相关的机械拉伸的重要性。
A variety of cells are subject to mechanical stretch in vivo, which plays a critical role in the function and homeostasis of cells, tissues, and organs. Deviations from the physiologically relevant mechanical stretch are often associated with organ dysfunction and various diseases. Although mechanical stretch is provided in some in vitro cell culture models, the effects of stretch dimensionality on cells are often overlooked and it remains unclear whether and how stretch dimensionality affects cell behavior. Here we develop cell culture platforms that provide 1-D uniaxial, 2-D circumferential, or 3-D radial mechanical stretches, which recapitulate the three major types of mechanical stretches that cells experience in vivo. We investigate the behavior of human microvascular endothelial cells and human alveolar epithelial cells cultured on these platforms, showing that the mechanical stretch influences cell morphology and cell-cell and cell-substrate interactions in a stretch dimensionality-dependent manner. Furthermore, the endothelial and epithelial cells are sensitive to the physiologically relevant 2-D and 3-D stretches, respectively, which could promote the formation of endothelium and epithelium. This study underscores the importance of recreating the physiologically relevant mechanical stretch in the development of in vitro tissue/organ models.