Restriction fragment length polymorphism analysis of reverse transcription-PCR products reveals the existence of two major strain groups of beet necrotic yellow vein virus.
Restriction fragment length polymorphism analysis of reverse transcription-PCR products reveals the existence of two major strain groups of beet necrotic yellow vein virus.
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逆转录PCR产物的限制性片段长度多态性分析揭示了甜菜坏死黄脉病毒存在两个主要毒株群。
DOI：
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发表时间：
1994
影响因子：
3.8
通讯作者：
W. Burgermeister
中科院分区：
文献类型：
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作者：
M. Kruse;R. Koenig;A. Hoffmann;A. Kaufmann;U. Commandeur;A. Solovyev;I. Savenkov;W. Burgermeister
甜菜坏死黄脉病毒 (BNYVV) 感染的甜菜是从欧洲许多地区以及亚洲和美国的一些地点获得的。针对病毒基因组的四个不同区域获得了超过 1 kbp 的逆转录 (RT)-PCR 产物，这对于病毒的致病特性（即 RNA 2 上的外壳蛋白和 42K 蛋白编码区以及 RNA 3 的主要部分）可能特别重要。 4. 这些 PCR 产物获得的限制性片段长度多态性 (RFLP) 模式揭示了 BNYVV 存在两个主要毒株群，分别为 A 型和 B 型。A 型在希腊、前南斯拉夫、斯洛伐克、奥地利、意大利、西班牙、法国、比利时、荷兰和英国的部分地区以及亚洲（土耳其、哈萨克斯坦、中国和日本）和美国检测到。B 型出现在德国和部分地区。法国。在A型和B型区域的交界处发现了混合感染。已发表的和新确定的 BNYVV 基因组各部分的核苷酸序列的比较表明，对于 RNA 2 和 3 的各自区域，A 型和 B 型之间的核苷酸差异百分比约为 3%，对于 RNA 4 约为 1.5%。核苷酸序列在两个毒株组中的每一个中似乎都非常稳定。 A 型和 B 型之间的大部分核苷酸差异发生在第三个三联体位置。外壳蛋白区域中的氨基酸变化位于病毒颗粒表面可接近的四个先前确定的抗原区域之外，并且参与连续和可能不连续表位的形成。这可以解释为什么两个菌株组之间没有发现血清学差异。
Beet necrotic yellow vein virus (BNYVV)-infected sugarbeets were obtained from many parts of Europe and also from some sites in Asia and the U.S.A. Reverse transcription (RT)-PCR products of more than 1 kbp were obtained for four different regions of the viral genome which may be particularly important with respect to the pathogenic properties of the virus, i.e. for the coat protein and the 42K protein-encoding regions on RNA 2 and for major parts of RNAs 3 and 4. Restriction fragment length polymorphism (RFLP) patterns obtained with these PCR products revealed the existence of two major strain groups of BNYVV, named type A and type B. The A type was detected in Greece, the former Yugoslavia, Slovakia, parts of Austria, Italy, Spain, parts of France, Belgium, The Netherlands and England as well as in Asia (Turkey, Kazachstan, China and Japan) and the U.S.A. The B type occurs in Germany and parts of France. Mixed infections were detected at the borderline regions between areas of the A and B types. Comparisons of published and newly determined nucleotide sequences of the respective parts of the BNYVV genome indicate that the percentage of nucleotide differences between the A and the B type is approximately 3% for the respective regions of RNAs 2 and 3 and approximately 1.5% for RNA 4. Nucleotide sequences appear to be remarkably stable within each of the two strain groups. The majority of the nucleotide differences between the A and B types occur in the third triplet position. The amino acid changes in the coat protein area are outside the four previously determined antigenic regions that are accessible on the surface of the virus particles and are involved in the formation of continuous and presumably also discontinuous epitopes. This may explain why serological differences between the two strain groups have not been found.