3-DIMENSIONAL RECONSTRUCTION OF SINGLE PARTICLES FROM RANDOM AND NONRANDOM TILT SERIES
3-DIMENSIONAL RECONSTRUCTION OF SINGLE PARTICLES FROM RANDOM AND NONRANDOM TILT SERIES
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DOI：
10.1002/jemt.1060090405
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发表时间：
1988-08-01
期刊：
影响因子：
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通讯作者：
RADERMACHER, M
中科院分区：
文献类型：
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作者：
RADERMACHER, M
为了克服辐射损伤引起的限制，从电子显微镜倾斜系列的三维重建的分辨率，新的重建方案已被开发，只需要一个单一的曝光的标本。用这些方法采集的倾斜序列具有随机投影方向。首先，描述了适用于从具有规则倾斜几何形状的倾斜系列获得的数据的三维重建技术，然后是这些技术的扩展，以允许分析具有随机间隔倾斜的投影系列。主要的重点放在加权反投影方法，最近已被扩展，以便适用于随机倾斜序列。除了对算法的描述之外，还解释了从单次曝光随机圆锥倾斜系列进行三维重建的完整过程，包括方位角的确定，圆锥倾斜系列的对准方案，可实现的分辨率对规则圆锥和单轴几何形状的投影数量的依赖性，以及使用相位残差和傅立叶环相关准则计算二维图像平均值和三维重建的实际分辨率的方法。给出了应用这些三维重建方法的生物样本的示例。
To overcome the radiation damage‐induced limitations to the resolution of three‐dimensional reconstructions from electron microscopic tilt series, novel reconstruction schemes have been developed that require only a single exposure of the specimen. The tilt series collected with these methods have random projection directions. First, three‐dimensional reconstruction techniques are described that are applicable to data obtained from tilt series with regular tilt geometry, followed by the extensions of these techniques to permit analysis of projection series with randomly spaced tilts. The main emphasis is placed on the weighted back‐projection methods, which have recently been extended so as to be applicable to random tilt series. Besides a description of the algorithms, the complete procedure for a threedimensional reconstruction from a single‐exposure, random conical tilt series is explained, including the determination of the azimuthal angles, the alignment scheme for conical tilt series, the dependence of the achievable resolution on the number of projections for regular conical and single‐axis geometries, and the method to calculate the actual resolution of two‐dimensional image averages and of three‐dimensional reconstructions using the phase residual and Fourier ring correlation criteria. Examples are given of biological specimens to which these three‐dimensional reconstruction methods have been applied.