Culturing Dermatophytes Rapidly from Each Toe Web by Fingertip
Culturing Dermatophytes Rapidly from Each Toe Web by Fingertip
复制标题
用指尖快速从每个脚趾网上培养皮肤癣菌
DOI：
10.1111/j.1346-8138.2005.tb00725.x
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
K. Nishioka
中科院分区：
文献类型：
--
作者：
Takao Sano;T. Katoh;K. Nishioka
通常通过显微镜检查和皮肤刮片培养来确诊。通过真菌培养分离皮肤癣菌提供了更可靠的感染证据，并具有鉴定致病微生物的优势。尽管如此，培养检查是对显微镜检查的补充，并且由于耗时和成本而不常规进行。在此，我们提出了一种新的培养方法，“手指取样法”，用于使用检查者的指尖作为样品收集工具从患者的趾蹼收集皮肤癣菌。使用这种方法，可以在一个培养皿上同时检查一只脚的四个趾蹼。对50例未经治疗的足跖进行了检查，107例KOH阳性足跖中83例（78%）有皮肤癣菌生长。手指取样法的分离率与熟练从业人员进行的传统皮肤刮片斜面培养的分离率相当。培养结果也呈阳性，19个53（36%）患病，但KOH阴性趾蹼和38（16%）正常趾蹼，表明存在不明感染。此外，我们证实了该方法用于检测刚刚暴露于污染区域的志愿者的健康趾蹼皮肤上的皮肤真菌附着的有效性，所述污染区域即日本公共浴室或被传播皮肤真菌繁殖体的患者踩过的浴垫。
The diagnosis of tinea pedis is usually confirmed by microscopy and culture of skin scrapings. Isolating dermatophytes by fungal culture gives more reliable proof of infection and has the advantage of identifying the causative organisms. Nevertheless, culture examination is complementary to microscopy and is not routinely performed because it is time‐ and cost‐consuming. Herein, we propose a new culturing method, the ‘finger‐sampling method‘, for collecting dermatophytes from patients' toe webs using the examiner's fingertip as a sample collection tool. Using this method, four toe webs of a foot can be examined at one time on one culture dish. Every toe web of 50 patients with untreated tinea pedis were examined, and dermatophytes were grown from 83 out of 107 (78%) KOH positive toe webs. The isolation ratio by the finger‐sampling method was comparable to that by traditional slant culture of skin scrapings performed by skilled practitioners. Culture results were also positive in 19 out of 53 (36%) diseased but KOH negative toe webs and in 38 (16%) normal toe webs, suggesting the existence of unidentified infection. Additionally, we confirmed the efficacy of this method for detecting dermatophyte attachment on the healthy toe web skin of volunteers who had just been exposed to contaminated areas, i.e. Japanese public baths or a bath mat stepped on by a patient disseminating dermatophyte propagules.