Purification and characterization of a monoclonal T-cell suppressor factor specific for poly(LGlu60LAla30LTyr10).
Purification and characterization of a monoclonal T-cell suppressor factor specific for poly(LGlu60LAla30LTyr10).
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聚 (LGlu60LAla30LTyr10) 特异性单克隆 T 细胞抑制因子的纯化和表征。
DOI：
10.1073/pnas.79.4.1254
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发表时间：
1982
影响因子：
11.1
通讯作者：
Webb,DR
中科院分区：
文献类型：
--
作者：
Krupen,K;Araneo,BA;Brink,L;Kapp,JA;Stein,S;Wieder,KJ;Webb,DR
对T细胞杂交瘤258 C4.4产生的三聚体聚(LGlu60LAla30LTyr10)特异的T细胞源性抑制因子进行了纯化。这是通过结合使用亲和层析和反相离子交换高效液相色谱分离培养基来完成的。纯化的因子由单一的MR 24000多肽链组成，均一的蛋白保持了对Poly(LGlu60LAla30LTyr10)的特异性抗体和T细胞增殖反应的抑制能力。纯抑制因子的比活度为8×10(7)单位/微克。
A monoclonal T-cell-derived suppressor factor specific for the terpolymer poly(LGlu60LAla30LTyr10) produced by the T-cell hybridoma 258 C4.4, was purified to homogeneity. This was accomplished by fractionation of the culture medium by using a combination of affinity chromatography and reverse-phase and ion-exchange high-performance liquid chromatography. The purified factor is composed of a single Mr 24,000 polypeptide chain, and the homogeneous protein maintains the ability to suppress antibody and T-cell proliferative responses to poly(LGlu60LAla30LTyr10) specifically. The specific activity of pure suppressor factor is calculated to be 8 X 10(7) units/micrograms.