Mechanistic insight into eukaryotic 60S ribosomal subunit biogenesis by cryo-electron microscopy.
Mechanistic insight into eukaryotic 60S ribosomal subunit biogenesis by cryo-electron microscopy.
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DOI：
10.1261/rna.057927.116
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发表时间：
2016-11
期刊：
影响因子：
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通讯作者：
Greber BJ
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文献类型：
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作者：
Greber BJ
真核核糖体是细胞的蛋白质生产工厂，由四个核糖体RNA分子和大约80种蛋白质组成。它们的生物发生是一个复杂的过程，涉及200多个生物发生因子，这些因子促进核糖体组分的产生、修饰和组装以及前核糖体颗粒沿着成熟途径的结构转变。在这里，我回顾了最近的结构和机制的见解，进一步通过冷冻电子显微镜原生纯化的前60 S颗粒和体外重建的核糖体组装因子复合物的大核糖体亚基的生物起源。结合生物化学，遗传和以前的结构数据，这些结构提供了详细的见解60 S亚基的中央突起的组装和成熟，核糖体隧道出口附近的生物发生因子的网络，以及在细胞质成熟过程中大核糖体亚基的功能激活。
Eukaryotic ribosomes, the protein-producing factories of the cell, are composed of four ribosomal RNA molecules and roughly 80 proteins. Their biogenesis is a complex process that involves more than 200 biogenesis factors that facilitate the production, modification, and assembly of ribosomal components and the structural transitions along the maturation pathways of the pre-ribosomal particles. Here, I review recent structural and mechanistic insights into the biogenesis of the large ribosomal subunit that were furthered by cryo-electron microscopy of natively purified pre-60S particles and in vitro reconstituted ribosome assembly factor complexes. Combined with biochemical, genetic, and previous structural data, these structures have provided detailed insights into the assembly and maturation of the central protuberance of the 60S subunit, the network of biogenesis factors near the ribosomal tunnel exit, and the functional activation of the large ribosomal subunit during cytoplasmic maturation.