CHEMOTAXIS OF RHIZOBIUM-MELILOTI TO THE PLANT FLAVONE LUTEOLIN REQUIRES FUNCTIONAL NODULATION GENES
CHEMOTAXIS OF RHIZOBIUM-MELILOTI TO THE PLANT FLAVONE LUTEOLIN REQUIRES FUNCTIONAL NODULATION GENES
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DOI：
10.1128/jb.170.7.3164-3169.1988
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发表时间：
1988-07-01
影响因子：
3.2
通讯作者：
BAUER, WD
中科院分区：
文献类型：
--
作者：
CAETANOANOLLES, G;CRISTESTES, DK;BAUER, WD
木犀草素是一种酚类化合物，在苜蓿根瘤菌中作为一种有效的和特异性的诱导剂，诱导苜蓿ABC基因的表达。我们发现R.苜蓿RCR 2011对木犀草素表现出正趋化性。在10-8 M时观察到最大趋化反应。两个密切相关的类黄酮，柚皮素和芹菜素，没有化学引诱剂。柚苷元的存在可抑制R.草木犀草素对木犀草素。一个大的删除在nif-nod区域的共生megaplasma消除了所有的趋化反应，但不影响一般的趋化性，如群大小的半软琼脂板和趋化性对脯氨酸在毛细管。转座子Tn 5突变在RbD、RbA或RbC中选择性地消除了R.草木犀草素到木犀草素。根癌农杆菌GMI 9050，一种缺乏Ti质粒的C58野生型的衍生物，对10-8 M毛地黄黄酮有化学反应。将含有290-糖苷酶nif-nod的DNA序列从R. meliloti转化为A.根癌农杆菌GMI 9050使受体能够对浓度峰值为10-6 M以及浓度峰值为10-8 M的毛地黄黄酮产生应答。A的反应。根癌农杆菌GMI 9050对木犀草素的抑制作用也被柚皮素的存在所消除。
Luteolin is a phenolic compound from plants that acts as a potent and specific inducer of nodABC gene expression in Rhizobium meliloti. We have found that R. meliloti RCR2011 exhibits positive chemotaxis towards luteolin. A maximum chemotactic response was observed at 10-8 M. Two closely related flavonoids, naringenin and apigenin, were not chemoattractants. The presence of naringenin but not apigenin abolished chemotaxis of R. meliloti towards luteolin. A large deletion in the nif-nod region of the symbiotic megaplasmid eliminated all chemotactic response to lueolin but did not affect general chemotaxis, as indicated by swarm size on semisoft agar plates and chemotaxis towards proline in capillary tubes. Transposon Tn5 mutations in nodD, nodA, or nodC selectively abolished the chemotactic response of R. meliloti to luteolin. Agrobacterium tumefaciens GMI9050, a derivative of the C58 wild type lacking a Ti plasmid, responded chemotactically to 10-8 M luteolin. The introduction of a 290-kilobase nif-nod-containing sequence of DNA from R. meliloti into A. tumefaciens GMI9050 enabled the recipient to respond to luteolin at concentrations peaking at 10-6 M as well as at concentrations peaking at 10-8 M. The response of A. tumefaciens GMI9050 to luteolin was also abolished by the presence of naringenin.