Direct gene transfer and expression into rat heart in vivo.
Direct gene transfer and expression into rat heart in vivo.
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发表时间：
1991
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影响因子：
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通讯作者：
G. Acsadi;Jiao Ss;Á. Jáni;D. Duke;P. Williams;W. Chong;J. Wolff
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作者：
G. Acsadi;Jiao Ss;Á. Jáni;D. Duke;P. Williams;W. Chong;J. Wolff
我们以前发现，基因注入骨骼肌可以采取的肌纤维和表达。在目前的研究中，我们发现心肌细胞也可以表达各种报告基因注入心肌作为有效的骨骼肌纤维，而其他几种组织的细胞不能。除横纹肌以外的组织不能表达注射的DNA不是由于注射的技术困难，因为通过PCR分析在这些其他组织中检测到注射的DNA。这些结果表明，骨骼肌和心肌细胞具有独特的功能，如T小管，可能在DNA摄取中发挥关键作用。心肌中的表达仅稳定了两周，可能是因为针对转染细胞的免疫应答。将基因直接转移到心肌细胞中的能力提高了获得性和遗传性心脏病基因治疗的可能性。
We found previously that genes injected into skeletal muscle can be taken up by myofibers and expressed. In the present study we found that myocardial cells can also express a variety of reporter genes injected into myocardium as efficiently as skeletal myofibers, while the cells of several other tissues cannot. The inability of tissues other than striated muscle to express injected DNA is not due to technical difficulties of injection because injected DNA was detected in these other tissues by PCR analysis. These results suggest that skeletal and cardiac muscle cells have unique features such as T tubules that may play a critical role in DNA uptake. Expression in cardiac muscle was stable for only two weeks, possibly because of an immune response against the transfected cells. The ability to directly transfer genes into myocardial cells raises the possibility of gene therapy for both acquired and genetic heart diseases.