The death mechanism of the harmful algal bloom species Alexandrium tamarense induced by algicidal bacterium Deinococcus sp. Y35.
The death mechanism of the harmful algal bloom species Alexandrium tamarense induced by algicidal bacterium Deinococcus sp. Y35.
复制标题
杀藻细菌奇异球菌诱导有害藻类塔玛亚历山大藻的死亡机制。
DOI：
10.3389/fmicb.2015.00992
复制
发表时间：
2015
影响因子：
5.2
通讯作者：
Zheng T
中科院分区：
文献类型：
--
作者：
Li Y;Zhu H;Lei X;Zhang H;Cai G;Chen Z;Fu L;Xu H;Zheng T
有害藻华（HABs）对水生生态系统造成各种有害影响，尤其是有毒的塔玛兰绿藻（Alexandrium tamarense），它通过向环境中释放麻痹性贝类毒素，对海洋经济和人类健康构成严重威胁。采用抑藻菌Deinococcus sp. Y35对tamarense进行抑藻作用机制研究。在灭藻作用下，测定了藻细胞的生长状态、活性氧含量、光合系统和核系统。菌株Y35的培养不仅在0.5 h内诱导了藻细胞ROS的过量产生，而且降低了总蛋白含量和抗氧化酶的反应。同时，脂质过氧化引起细胞膜完整性丧失。叶绿素a和类胡萝卜素等光合色素含量下降，光合效率受到明显抑制。同时，光合作用相关基因表达下调。此外，还证实了破坏细胞核结构和抑制增殖细胞核抗原（PCNA）相关基因的表达。研究了该杀藻菌对褐藻的潜在作用机制，为藻华的防治提供了新的思路。
Harmful algal blooms (HABs) cause a variety of deleterious effects on aquatic ecosystems, especially the toxic dinoflagellate Alexandrium tamarense, which poses a serious threat to marine economic and human health based on releasing paralytic shellfish poison into the environment. The algicidal bacterium Deinococcus sp. Y35 which can induce growth inhibition on A. tamarense was used to investigate the functional mechanism. The growth status, reactive oxygen species (ROS) content, photosynthetic system and the nuclear system of algal cells were determined under algicidal activity. A culture of strain Y35 not only induced overproduction of ROS in algal cells within only 0.5 h of treatment, also decrease the total protein content as well as the response of the antioxidant enzyme. Meanwhile, lipid peroxidation was induced and cell membrane integrity was lost. Photosynthetic pigments including chlorophyll a and carotenoid decreased along with the photosynthetic efficiency being significantly inhibited. At the same time, photosynthesis-related gene expression showed down-regulation. More than, the destruction of cell nuclear structure and inhibition of proliferating cell nuclear antigen (PCNA) related gene expression were confirmed. The potential functional mechanism of the algicidal bacterium on A. tamarense was investigated and provided a novel viewpoint which could be used in HABs control.