Studies of anti-inflammatory effects of Rooibos tea in rats
Studies of anti-inflammatory effects of Rooibos tea in rats
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DOI：
10.1111/j.1442-200x.2009.02835.x
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发表时间：
2009-10-01
影响因子：
1.4
通讯作者：
Shimizu, Toshiaki
中科院分区：
文献类型：
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作者：
Baba, Haruna;Ohtsuka, Yoshikazu;Shimizu, Toshiaki
背景：众所周知，路易波士茶不含咖啡因，含有丰富的类黄酮。路易波士茶中主要的黄酮类化合物Aspalathin和nothofagin具有较强的抗氧化活性，由于氧化应激可诱导炎症反应，本研究采用大鼠结肠炎模型研究路易波士茶的抗炎作用。经过4周的繁殖，血清超氧化物歧化酶（SOD）水平测定使用电子自旋共振分析。尿8-羟基-2 '-脱氧鸟苷（8-OHdG）浓度也被确定为使用酶联免疫吸附试验的DNA损伤的反映。此外，向大鼠施用葡聚糖硫酸钠（DSS），已知其在啮齿动物中诱导结肠炎，有或没有路易波士茶以评估其抗炎活性。结果：两组患者的体重增长和实验室指标无显著性差异，两组患者的临床症状、血红蛋白、血清铁和超氧化物歧化酶（SOD）水平也无显著性差异。Rooibos组血清SOD水平显著高于对照组（P <0.05），尿8-羟基-2 '-脱氧鸟苷水平显著低于对照组（P < 0.05）。给药后，路易波士组血清SOD水平显著高于对照组（P < 0.05）。其结果是，血红蛋白水平下降，观察到在对照组中，防止在路易波士组后的DSS challenge.Conclusion：路易波士茶可以防止DNA损伤和炎症，其抗氧化活性在体内。由于路易波士茶不含咖啡因，因此常规摄入可能是安全的，有助于减少儿童的氧化应激。
Background:Rooibos tea is known to be caffeine free with abundant flavonoids. Aspalathin and nothofagin, the main flavonoids contained in Rooibos tea, have stronger anti-oxidative activity than other flavonoids.As oxidative stress can induce inflammation, the anti-inflammatory effects of Rooibos tea were investigated using a rat colitis model.Methods:Seven-week-old Wister rats were divided into two groups: one group given Rooibos tea, and one given water. After four weeks of breeding, serum superoxide dismutase (SOD) levels were determined using the Electron Spin Resonance analysis. Urine 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations were also determined as reflections of DNA damage using enzyme-linked immunosorbent assay. Furthermore, rats were administrated dextran sodium sulfate (DSS), which is known to induce colitis in rodents, with or without Rooibos tea to evaluate its anti-inflammatory activity. Clinical symptoms, hemoglobin, serum iron and SOD levels were compared between the groups.Results:There were no significant differences in bodyweight gain or laboratory data between the groups. The serum SOD levels were significantly increased, and urine 8-hydroxy-2'-deoxyguanosine levels were significantly decreased in the Rooibos group compared with the controls (P < 0.05 in each). After DSS administration, the serum SOD levels were significantly higher in the Rooibos group compared to the controls (P < 0.05). As a result, a decreased hemoglobin level, observed in the control group, was prevented in the Rooibos group after the DSS challenge.Conclusion:Rooibos tea may prevent DNA damage and inflammation by its anti-oxidative activity in vivo. As Rooibos tea is free from caffeine, routine intake may be safe and useful in reducing oxidative stress in children.