Helminth-induced CD9+ B-cell subset alleviates obesity-associated inflammation via IL-10 production
Helminth-induced CD9+ B-cell subset alleviates obesity-associated inflammation via IL-10 production
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DOI：
10.1016/j.ijpara.2021.08.009
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发表时间：
2022-02-04
影响因子：
4
通讯作者：
Ji, Minjun
中科院分区：
文献类型：
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作者：
Li, Maining;Wang, Huiquan;Ji, Minjun
流行病学调查和动物实验表明，蠕虫感染可以在一定程度上预防肥胖并提高胰岛素敏感性。与此同时，蠕虫会诱导形成调节性免疫细胞网络，包括调节性 B 细胞 (Breg)。然而，这些 Bregs 在改善全身代谢稳态方面的分子特征和功能仍不清楚。我们建立了慢性日本血吸虫感染的小鼠模型，并使用RNA-seq比较了感染小鼠脾B细胞中B10细胞（CD19+CD5+CD1dhi）和B10-细胞（CD19+CD5-CD1d-）的差异。确定了一个独特的 Breg 群体。此外，还评估了这些 Bregs 在体外产生抑制性细胞因子的能力，以及在过继转移到高脂肪饮食小鼠体内时抑制肥胖的能力。我们发现血吸虫感染可以扩大小鼠的 Breg 细胞群。 CD9 被证明是脾脏中大多数小鼠 IL-10+ B 细胞的关键表面标记。 CD19+CD9+ B 细胞比传统 B10 细胞产生更多的 IL-10。 CD9+ B 细胞的过继转移能够通过促进高脂饮食小鼠中的 Tregs、Th2 细胞和减少 Th1、Th17 细胞来减轻肥胖相关的炎症。总之，血吸虫感染可以诱导调节性 CD9+ B 细胞产生，这在通过 IL-10 产生调节代谢紊乱中发挥着关键作用。 (c) 2021 年澳大利亚寄生虫学会。由爱思唯尔有限公司出版。保留所有权利。
It has been shown that helminth infection can protect against obesity and improve insulin sensitivity to a certain extent, based on epidemiological investigations and animal experiments. Meanwhile, helminths induce a network of regulatory immune cells, including regulatory B cells (Bregs). However, the molecule characteristics and function of these Bregs in improving whole-body metabolic homeostasis remains largely unclear. We established a mouse model with chronic Schistosoma japonicum infection, and compared the differences in B10 cells (CD19+CD5+CD1dhi) and B10- cells (CD19+CD5-CD1d-) from splenic B cells of infected mice using RNA-seq. A unique Breg population was identified. Furthermore, these Bregs were evaluated for their ability to produce inhibitory cytokines in vitro and suppress obesity when adoptively transferred into mice on a high-fat diet. We found that schistosome infection could expand Breg cell populations in mice. CD9 was demonstrated to be a key surface marker for most murine IL-10+ B cells in spleen. CD19+CD9+ B cells produced more IL-10 than conventional B10 cells. Adoptive transfer of CD9+ B cells had the capacity to alleviate obesity-associated inflammation via promoting Tregs, Th2 cells and decreasing Th1, Th17 cells in high-fat diet mice. In conclusion, schistosome infection can induce regulatory CD9+ B cell production, which plays a critical role in the regulation of metabolic disorders through IL-10 production. (c) 2021 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.