Karyotyping and single-gene detection using fluorescence in situ hybridization on chromosomes of Hydra magnipapillata (Cnidaria: Hydrozoa)
Karyotyping and single-gene detection using fluorescence in situ hybridization on chromosomes of Hydra magnipapillata (Cnidaria: Hydrozoa)
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DOI：
10.3897/compcytogen.v4i2.41
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发表时间：
2010-01-01
影响因子：
1
通讯作者：
Bosch, T. C. G.
中科院分区：
文献类型：
--
作者：
Anokhin, B.;Hemmrich-Stanisak, G.;Bosch, T. C. G.
淡水水母Hydra L.，1758(线虫纲，水母纲)是现代进化和发育生物学中的重要模式生物。一个完整的基因组序列最近已经被公布，1947年，分子数据正在迅速积累在文献中，但关于它的染色体的信息很少。在这项研究中，描述了一种高效的荧光原位杂交(FISH)方法，该方法不仅可以识别染色体，还可以可视化单个遗传座位的位置。结合cDNA和基因组测序，这可能为在这种基本的后生动物模式生物中进行越来越精确的遗传和物理定位提供基础。
The fresh water polyp Hydra L., 1758 (Cnidaria, Hydrozoa) plays a key role as a model organism in modern evolutionary and developmental biology. A complete genome sequence has been published recently for Hydra magnipapillata Ito, 1947 and molecular data are rapidly accumulating in the literature, but little information is available on its chromosomes. In this study, an efficient fluorescence in situ hybridization (FISH) method is described for H. magnipapillata which not only allows identification of the chromosomes but also visualization of the location of individual genetic loci. Together with cDNA and genomic sequencing this may provide the foundation for increasingly precise genetic and physical mapping in this basal metazoan model organism.