THE CAULIFLOWER MOSAIC VIRUS-35S PROMOTER - COMBINATORIAL REGULATION OF TRANSCRIPTION IN PLANTS
THE CAULIFLOWER MOSAIC VIRUS-35S PROMOTER - COMBINATORIAL REGULATION OF TRANSCRIPTION IN PLANTS
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DOI：
10.1126/science.250.4983.959
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发表时间：
1990-11-16
期刊：
影响因子：
56.9
通讯作者：
CHUA, NH
中科院分区：
文献类型：
--
作者：
BENFEY, PN;CHUA, NH
高等植物转录的适当调控需要基因调控区域的特定顺式元件及其相应的反式作用蛋白。花椰菜花叶病毒（CaMV） 35s启动子的分析有助于了解其转录调控机制。在大多数植物中，完整的35s启动子在异源基因上具有组成性表达。解剖到能够赋予组织特异性基因表达的子域已经证明启动子具有模块化组织。当选定的子结构域组合在一起时，它们赋予了从孤立的子结构域中检测不到的表达，这表明顺式元件之间发生了协同相互作用。烟草和矮牵牛中35s子结构域的特定组合所赋予的表达模式不同。这表明顺式调控元件的组合编码在不同的物种中可能有不同的解释。
Appropriate regulation of transcription in higher plants requires specific cis elements in the regulatory regions of genes and their corresponding trans-acting proteins. Analysis of the cauliflower mosaic virus (CaMV) 35Spromoter has contributed to the understanding of transcriptional regulatory mechanisms. The intact 35Spromoter confers constitutive expression upon heterologous genes in most plants. Dissection into subdomains that are able to confer tissue-specific gene expression has demonstrated that the promoter has a modular organization. When selected subdomains are combined, they confer expression not detected from the isolated subdomains, suggesting that synergistic interactions occur among cis elements. The expression patterns conferred by specific combinations of 35Ssubdomains differ in tobacco and petunia. This indicates that a combinatorial code of cis-regulatory elements may be interpreted differently in different species.