Exogenous calcium alleviates low night temperature stress on the photosynthetic apparatus of tomato leaves.

Exogenous calcium alleviates low night temperature stress on the photosynthetic apparatus of tomato leaves.
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外源钙缓解夜间低温对番茄叶片光合机构的胁迫

DOI:
10.1371/journal.pone.0097322
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发表时间:
2014
期刊:
影响因子:
3.7
通讯作者:
Li T
Li T
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Zhang G;Liu Y;Ni Y;Meng Z;Lu T;Li T

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研究了外源CaCl2对低夜间温度(LNT)下番茄叶片光系统I和II(PSI和PSII)活性、循环电子流(CEF)和质子动力的影响。 LNT胁迫降低了净光合速率(Pn)、PSII有效量子产率[Y(II)]和光化学猝灭(qP),而CaCl2预处理提高了LNT胁迫下的Pn、Y(II)和qP。 LNT 应力显着增加了能量耗散 [Y(NO)] 的非调节量子产率,而 CaCl2 缓解了这种增加。外源 Ca2+ 增强 LNT 应激对 CEF 的刺激。 CaCl2 预处理减轻了 LNT 应激引起的氧化 PQ 库的抑制。 LNT 胁迫降低了玉米黄质的形成和 ATP 酶活性,但 CaCl2 预处理逆转了这两种影响。 LNT 应激导致类囊体膜上质子梯度的过度形成,而 CaCl2 预处理降低了 LNT 下的上述因素。因此,我们的结果表明,CaCl2 预处理可以减轻 LNT 胁迫植物的光抑制。我们的研究结果进一步表明,这种缓解部分是通过碳固定能力、PQ 库、线性和循环电子传输、叶黄素循环和 ATP 酶活性的改善来介导的。
The effect of exogenous CaCl2 on photosystem I and II (PSI and PSII) activities, cyclic electron flow (CEF), and proton motive force of tomato leaves under low night temperature (LNT) was investigated. LNT stress decreased the net photosynthetic rate (Pn), effective quantum yield of PSII [Y(II)], and photochemical quenching (qP), whereas CaCl2 pretreatment improved Pn, Y(II), and qP under LNT stress. LNT stress significantly increased the non-regulatory quantum yield of energy dissipation [Y(NO)], whereas CaCl2 alleviated this increase. Exogenous Ca2+ enhanced stimulation of CEF by LNT stress. Inhibition of oxidized PQ pools caused by LNT stress was alleviated by CaCl2 pretreatment. LNT stress reduced zeaxanthin formation and ATPase activity, but CaCl2 pretreatment reversed both of these effects. LNT stress caused excess formation of a proton gradient across the thylakoid membrane, whereas CaCl2 pretreatment decreased the said factor under LNT. Thus, our results showed that photoinhibition of LNT-stressed plants could be alleviated by CaCl2 pretreatment. Our findings further revealed that this alleviation was mediated in part by improvements in carbon fixation capacity, PQ pools, linear and cyclic electron transports, xanthophyll cycles, and ATPase activity.
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