Endothelial Progenitor Cells: Procedure for Cell Isolation and Applications
Endothelial Progenitor Cells: Procedure for Cell Isolation and Applications
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DOI：
10.1007/978-1-4939-6756-8_7
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发表时间：
2017-01-01
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影响因子：
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通讯作者：
Kishore, Raj
中科院分区：
文献类型：
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作者：
Garikipati, Venkata N. S.;Kishore, Raj
骨髓内皮祖细胞 (EPC) 在多种动物模型中显示出促进缺血组织新生血管形成和减轻缺血性损伤的巨大前景，这导致基于 EPC 的临床试验取得了适度但有希望的结果。 EPC 使用中的一些变量与其差异分离和表征方案有关，因为 EPC 文献并未确定这些血管祖细胞的独特标记。在本章中，我们介绍了 EPC 分离的分步方案、它们的表征和培养条件，以及它们在基础和临床研究中的潜在用途。
Bone marrow endothelial progenitor cells (EPCs) have shown a great promise to promote ischemic tissue neovascularization and to attenuate ischemic injury in a variety of animal models, which led to EPC-based clinical trials that yielded modest but promising results. Some of the variables in the use of EPCs relate to their differential isolation and characterization protocols since the EPC literature does not identify a unique marker for these vascular progenitors. In this chapter, we present step-by-step protocols for the isolation of EPCs, their characterization and culture conditions, and their potential use in basic and clinical research.