TNF-α influences the lateral dynamics of TNF receptor I in living cells
TNF-α influences the lateral dynamics of TNF receptor I in living cells
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DOI：
10.1016/j.bbamcr.2012.06.026
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发表时间：
2012-10-01
影响因子：
5.1
通讯作者：
Heilemann, Mike
中科院分区：
文献类型：
--
作者：
Heidbreder, Meike;Zander, Christin;Heilemann, Mike
在哺乳动物细胞中，炎症主要是通过肿瘤坏死因子α与肿瘤坏死因子受体1的结合而介导的。在本研究中，我们使用高密度单粒子跟踪结合光活化定位显微镜研究了配体结合前后肿瘤坏死因子-R1的横向动力学。我们的单分子数据表明，肿瘤坏死因子受体1存在于质膜上，具有不同的迁移率，表明存在不同的分子组织。胆固醇耗竭导致慢受体种类减少，平均扩散系数显著增加。此外，肿瘤坏死因子-α治疗的结果是，平均扩散系数适度增加，而其分布变窄。在我们观察的基础上，我们提出了肿瘤坏死因子受体1在质膜上的结构排列和激活的精细机制。(C)2012爱思唯尔B.V.保留所有权利。
In mammalian cells, inflammation is mainly mediated by the binding of tumor necrosis factor alpha to tumor necrosis factor receptor 1. In this study, we investigated lateral dynamics of TNF-R1 before and after ligand binding using high-density single-particle tracking in combination with photoactivated localization microscopy. Our single-molecule data indicates the presence of tumor necrosis factor receptor 1 with different mobilities in the plasma membrane, suggesting different molecular organizations. Cholesterol depletion led to a decrease of slow receptor species and a strong increase in the average diffusion coefficient. Moreover, as a consequence of tumor necrosis factor-alpha treatment, the mean diffusion coefficient moderately increased while its distribution narrowed. Based on our observation, we propose a refined mechanism on the structural arrangement and activation of tumor necrosis factor receptor 1 in the plasma membrane. (C) 2012 Elsevier B.V. All rights reserved.