Newly found Tetrahymena nucleoporins, Nup214, Nup153 and Pom121/Pom82, differentiate nuclear pore complexes of functionally distinct nuclei
Newly found Tetrahymena nucleoporins, Nup214, Nup153 and Pom121/Pom82, differentiate nuclear pore complexes of functionally distinct nuclei
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DOI：
10.1080/19420889.2017.1384890
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发表时间：
2017-11-13
影响因子：
--
通讯作者：
Haraguchi T
中科院分区：
文献类型：
--
作者：
Iwamoto M;Hiraoka Y;Haraguchi T
核孔复合物（NPC）是真核生物在细胞核和细胞质之间进行分子运输的唯一通道。鼻咽癌由大约30种不同的称为核孔蛋白的蛋白质组成。NPC的功能结构在各种真核生物中高度保守。然而，许多核孔蛋白发挥其特定功能的确切机制仍不清楚。单细胞纤毛虫四膜虫有两个功能不同的核，一个大核和一个微核，我们发现这两个核具有组成不同的NPCs。我们最初鉴定了四种核特异性Nup98-s，并证明它们是大核和微核特异性核转运所必需的。最近，我们发现了两个核特异性的Nup214和Nup153的类似物，以及两个核特异性的跨膜蛋白Pom121和Pom82。我们的研究结果表明，Nup214， Nup153和Pom121/Pom82也作为转运机制的关键分子，以区分存在于该生物体中的两种功能不同的细胞核。
The nuclear pore complex (NPC) is the sole gateway for molecular transport between the nucleus and the cytoplasm in eukaryotes. The NPC is composed of approximately 30 different kinds of protein components called nucleoporins. The functional structure of the NPC is highly conserved among various eukaryotes. However, the exact mechanisms by which many of the nucleoporins exert their specific functions still remain unclear. The single cell ciliate Tetrahymena has two functionally distinct nuclei, a macronucleus and a micronucleus, and we have discovered that these two nuclei have compositionally distinct NPCs. We initially identified four nucleus-specific Nup98-s and demonstrated that they are required for macronucleus- and micronucleus-specific nuclear transport. More recently we identified two nucleus-specific paralogs of Nup214 and Nup153 and two nucleus-specific transmembrane proteins Pom121 and Pom82. Our findings argue that Nup214, Nup153, and Pom121/Pom82 also act as key molecules for transport machineries to differentiate the two functionally distinct nuclei present in this organism.