SPATIAL AND FUNCTIONAL CORRELATION BETWEEN DIAMINE-OXIDASE AND PEROXIDASE-ACTIVITIES AND THEIR DEPENDENCE UPON DEETIOLATION AND WOUNDING IN CHICKPEA STEMS
SPATIAL AND FUNCTIONAL CORRELATION BETWEEN DIAMINE-OXIDASE AND PEROXIDASE-ACTIVITIES AND THEIR DEPENDENCE UPON DEETIOLATION AND WOUNDING IN CHICKPEA STEMS
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DOI：
10.1007/bf00239989
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发表时间：
1990-08-01
期刊：
影响因子：
4.3
通讯作者：
FEDERICO, R
中科院分区：
文献类型：
--
作者：
ANGELINI, R;MANES, F;FEDERICO, R
沿着测定了光生鹰嘴豆茎中二胺氧化酶（DAO，EC www.example.com）和过氧化物酶（POD，EC www.example.com）的活性。（鹰嘴豆）幼苗。酶活性进行了评价，在可溶性，轻度结合（盐提取）和紧密结合（Driselase消化）壁馏分，并在残留馏分从不同的节间。通过组织化学和免疫组织化学技术，酶和木质素沉积的表观组织分布进行了可视化。茎沿着可溶性组分和壁组分中DAO和POD活性之间存在密切关系。这两种酶的生化活性从茎的基部到顶端呈下降趋势，与木质化组织在茎中的分布规律一致。沿着沿着整个器官的表皮，发现每种酶都有类似的活性梯度。此外，脱黄化引起了这两种酶在该组织中的活动上升。受伤鹰嘴豆茎诱导的DAO和POD活性的可溶性和壁馏分平行增加。两种酶的原位组织化学检测表明，平行发生的DAO/POD系统和木质素化沉积在邻近伤口部位的细胞壁。通过淀粉凝胶电泳测定了创伤愈合过程中POD同工型的模式。除了可溶性组分和壁组分中酶带相对强度的变化外，可溶性组分中还出现了一种新的POD同工型，可能与损伤反应有关。这种异构体被证明是轻微结合到细胞壁，因为它可以检测到从伤口愈合的幼苗获得的细胞外液。上述结果的基础上，一个严格的空间和功能的相关性，可以推断之间的DAO和POD鹰嘴豆，并可能在其他豆科植物物种，根据以前的证据表明这些酶的质外体中的综合作用。
The activities of diamine oxidase (DAO, EC 1.4.3.6) and peroxidase (POD, EC 1.11.1.7) were determined along the stems of light-grown Cicer arietinum L. (chick-pea) seedlings. Enzyme activities were evaluated in the soluble, lightly bound (salt extraction) and tightly bound (Driselase digestion) wall fractions, and in residual fractions obtained from the different internodes. Apparent tissue distributions of both enzymes and lignin depositions were visualised by means of histochemical and immunohistochemical techniques. A close relationship was found between DAO and POD activities in the soluble and wall fractions along the stem. The biochemical activities of both enzymes decreased from the base to the apex of the stem in parallel with the distribution pattern of lignifying tissues in this organ. A similar activity gradient was found for each enzyme along the epidermis of the whole organ. Moreover, deetiolation elicited a rise in the activities of both enzymes in this tissue. Wounding chick-pea stems induced parallel increases in DAO and POD activities in the soluble and wall fractions. In-situ histochemical detection of both enzymes demonstrated the parallel occurrence of the DAO/POD system and lignosuberised depositions in the cell walls adjacent to the wound site. The patterns of POD isoforms resulting from the wound-healing process were determined by means of starch-gel electrophoresis. In addition to changes in relative intensity of enzyme bands in soluble and wall fractions, a new POD isoform, possibly related to the wounding response, appeared in the soluble fraction. This isoform was shown to be lightly bound to cell walls as it could be detected in the extracellular fluids obtained from wound-healed seedlings. On the basis of the above-mentioned results, a strict spatial and functional correlation can be inferred between DAO and POD in chick-pea, and probably in other Leguminosae species, in accordance with previous evidence indicating an integrated role for these enzymes in the apoplast.