Infiltration of CD14-positive macrophages at the invasive front indicates a favorable prognosis in colorectal cancer patients with lymph node metastasis.
Infiltration of CD14-positive macrophages at the invasive front indicates a favorable prognosis in colorectal cancer patients with lymph node metastasis.
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发表时间：
2011-03
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通讯作者：
M. Kinouchi;K. Miura;T. Mizoi;K. Ishida;W. Fujibuchi;T. Ando;Nobuki Yazaki;Kazuya Saito;Ken‐ichi Shiiba;I. Sasaki
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作者：
M. Kinouchi;K. Miura;T. Mizoi;K. Ishida;W. Fujibuchi;T. Ando;Nobuki Yazaki;Kazuya Saito;Ken‐ichi Shiiba;I. Sasaki
背景/目的应用流式细胞术和免疫组化技术研究CD 14+巨噬细胞在结直肠癌中的分布，探讨CD 14+巨噬细胞在结直肠癌中的作用。方法：对52例结直肠癌患者癌组织和相应正常组织中CD 14+、CD 1a+、CD 83+和CD 68+细胞的分布进行研究，并与临床病理特征及T细胞活化共刺激因子CD 14+巨噬细胞中CD 86和CD 80的表达进行相关性分析。结果1）CD 14+巨噬细胞主要分布在大肠癌组织的浸润前沿，而非正常组织; 2）CD 14+巨噬细胞表达CD 86和CD 80的比例较高; 3）在有淋巴结转移的大肠癌病例中，高CD 14组的5年总生存率其中CD 14+巨噬细胞数高于中位数者，优于低CD 14组。结论CD 140巨噬细胞浸润在结直肠癌淋巴结转移灶前沿，提示结直肠癌淋巴结转移患者预后良好。此外，CD 14+巨噬细胞和T细胞的活化可能有助于为结直肠癌患者开发新的免疫策略。
BACKGROUND/AIMS To clarify the distribution of CD14+ macrophages in colorectal cancer using flow cytometry and immunohistochemistry, and to elucidate the roles of CD14+ macrophages in colorectal cancer. METHODOLOGY We studied the paired cancerous and corresponding normal tissues from 52 patients with colorectal cancer for the distribution of CD14+, CD1a+, CD83+ and CD68+ cells, and correlated the findings with the clinicopathological characteristics and with the expression of CD86 and CD80 in the CD14+ macrophages, which are co-stimulatory factors for T cell activation. RESULTS 1) CD14+ macrophages were distributed predominantly at the invasive front of colorectal cancer tissues, rather than in the normal tissues, 2) a high percentage of the CD14+ macrophages expressed CD86 and CD80, and 3) in the colorectal cancer cases with lymph node metastasis, the 5-year overall survival rate of the high CD14 group, in which the number of CD14+ macrophages was higher than the median, was better than that of the low CD14 group. CONCLUSION The infiltration of CD140 macrophages at the invasive front indicates a favorable prognosis in colorectal cancer patients with lymph node metastasis. In addition, the activation of CD14+ macrophages and T cells may facilitate the development of new immunotherapeutic strategies for colorectal cancer patients.