Amplified fragment length polymorphisms and sequence data in the phylogenetic analysis of polyploids:: multiple origins of Veronica cymbalaria (Plantaginaceae)
Amplified fragment length polymorphisms and sequence data in the phylogenetic analysis of polyploids:: multiple origins of Veronica cymbalaria (Plantaginaceae)
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DOI：
10.1111/j.1469-8137.2007.02172.x
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发表时间：
2007-01-01
期刊：
影响因子：
9.4
通讯作者：
Albach, Dirk C.
中科院分区：
文献类型：
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作者：
Albach, Dirk C.
多倍体婆婆纳的起源利用DNA序列数据和扩增片段长度多态性（AFLP）指纹技术研究了该分类群的亲缘关系。AFLP指纹技术在系统发育分析中的应用是有问题的，因此比较了各种方法。DNA序列数据（对于内部转录间隔区（ITS）区域和质体trnL-F区域，（trnL内含子，3 '外显子，和trnL-F间隔区））和聚合酶链反应-限制性片段长度多态性ITS区的PCR-RFLP分析表明，该复合体的进化可能是一个可靠的假说。这一假设允许不同的距离措施（Jaccard和内李）在表型，字符状态加权简约性，和贝叶斯分析的AFLP Marker.The研究建立的效果进行评估，四倍体V. cymbalaria起源于地中海东部至少两次，在两个不同的起源与父母之一。六倍体V. cymbalaria起源更频繁。结果表明，即使AFLP标记分析中的细微差异也会导致截然不同的结论。这项研究揭示了地中海多倍体物种的多个起源。此外，它表明，分析一个复杂的标记系统，如AFLP指纹只使用一种类型的分析很容易产生误导。
The origin of polyploid Veronica cymbalaria (Plantaginaceae) was investigated using DNA sequence data and amplified fragment length polymorphism (AFLP) fingerprints to reveal the parentage of this taxon. The use of AFLP fingerprints in phylogenetic analysis is problematic and various methods have therefore been compared.DNA sequence data (for the internal transcribed spacer (ITS) region and the plastid trnL-F region (trnL intron, 3'exon, and trnL-F spacer)) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the ITS region suggested a reliable hypothesis for the evolution of the V. cymbalaria complex. This hypothesis allowed evaluation of the effect of different distance measures (Jaccard and Nei-Li) in phenetic, character-state weighted parsimony, and Bayesian analyses of AFLP markers.The study establishes that tetraploid V. cymbalaria originated at least twice in the eastern Mediterranean, with one parent differing in the two separate origins. Hexaploid V. cymbalaria originated even more often. The results illustrate that even subtle differences in the analyses of AFLP markers can lead to drastically different conclusions.The study reveals multiple origins of a Mediterranean polyploid species. Furthermore, it demonstrates that the analysis of a complex marker system such as AFLP fingerprints using only one type of analysis can easily be misleading.