Molecular Epidemiology of Paramyxoviruses in Frugivorous Eidolon helvum Bats in Zambia
Molecular Epidemiology of Paramyxoviruses in Frugivorous Eidolon helvum Bats in Zambia
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DOI：
10.1292/jvms.13-0518
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发表时间：
2014-04-01
影响因子：
1.2
通讯作者：
Sawa, Hirofumi
中科院分区：
文献类型：
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作者：
Muleya, Walter;Sasaki, Michihito;Sawa, Hirofumi
在这项研究中，我们描述了从果蝠的Eidolon helvum种中检测到的新型副粘病毒。我们从4年间（2008-2011年）在赞比亚捕获的312只蝙蝠的脾脏样本中提取了RNA。半巢式RT-PCR共检测到25份（8%）副粘病毒阳性样本，并对其进行直接测序和系统发育分析。在阳性样本中检出7种新型副粘病毒。5种病毒与亨尼帕病毒属密切相关，2种病毒与来自加纳和刚果布拉柴维尔的未分类蝙蝠副粘病毒相关。本研究利用RT-PCR技术在Kansaka国家公园的果蝠中发现了与亨尼帕病毒相关和不相关的新型病毒，并表明存在来自广泛地理区域的类似蝙蝠副粘病毒，这表明蝙蝠具有携带和传播病毒的能力。果蝠体内存在这些病毒可能会对公众健康构成威胁。
In this study, we describe the detection of novel paramyxoviruses from the Eidolon helvum species of fruit bats. We extracted RNA from 312 spleen samples from bats captured in Zambia over a period of 4 years (2008-2011). Semi-nested RT-PCR detected a total of 25 (8%) positive samples for paramyxoviruses which were then directly sequenced and analyzed using phylogenetic analysis. Among the positive samples, seven novel paramyxoviruses were detected. Five viruses were closely related to the genus Henipavirus, while two viruses were related to the unclassified Bat paramyxoviruses from Ghana and Congo Brazzaville. Our study identified novel Henipavirus-related and unrelated viruses using RT-PCR in fruit bats from Kansaka National Park and indicated the presence of similar Bat paramyxoviruses originating from wide geographic areas, suggesting the ability of bats to harbor and transmit viruses. The presence of these viruses in fruit bats might pose a public health risk.