Biocontrol of Fusarium sambucinum, dry rot of potato, by Serratia plymuthica 5-6
Biocontrol of Fusarium sambucinum, dry rot of potato, by Serratia plymuthica 5-6
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DOI：
10.1080/09583150802478189
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发表时间：
2008-01-01
影响因子：
1.4
通讯作者：
Hynes, Russell K.
中科院分区：
文献类型：
--
作者：
Gould, Marilyn;Nelson, Louise M.;Hynes, Russell K.
从豌豆（Pisum sativum（L））根际分离的Serratia grimesii 4-9和Serratia lymuthica 5-6在体外抑制接骨木镰刀菌（Fusarium sambucinum）生长和减少贮藏马铃薯（Solanum tuberosum L）中镰刀菌干腐病的潜力进行了评价。体外研究表明，这些细菌分离物抑制F。在15和25摄氏度下，Sambucinum增加60%或更多。在马铃薯块茎切片检测中，暴露于F. Sambucinum和S. grimesii 4-9和S.在15摄氏度下，Almuthica 5-6分别减少了96%和97%。感染部位的直径（mm）分别减少了91%和96%，与F处理的切片相比。单用接骨木对镰刀菌感染的整个马铃薯块茎的研究也表明，用细菌分离物或杀真菌剂噻菌灵处理后，干腐病的形成显著减少。当与病原菌同时施用时，S. grimesii 4-9和S.与单独用病原体接种的块茎相比，在15 ° C下，Alummuthica 5-6分别抑制镰刀菌干腐病的发展60%和77%，在25 ° C下分别抑制63%和84%。噻菌灵抑制镰刀菌干腐病的发展，分别为66%和81%，在15和25摄氏度，与单独接种的病原体相比。这些研究表明土壤细菌作为生物杀真菌剂用于管理收获后作物病害的潜力。由于与S. grimesii 4-9，S.建议将L5 -6作为生物杀菌剂开发的研究对象。
Serratia grimesii 4-9 and Serratia plymuthica 5-6, isolated from the rhizosphere of pea, Pisum sativum (L), were evaluated for their potential to suppress growth of Fusarium sambucinum in vitro and to reduce Fusarium dry rot in stored potatoes (Solanum tuberosum L). In vitro studies indicated that these bacterial isolates suppressed growth of F. sambucinum by 60% or more at both 15 and 25 degrees C. In a potato tuber slice assay the number of infection sites in potato slices exposed to F. sambucinum and treated with S. grimesii 4-9 and S. plymuthica 5-6 was reduced by 96 and 97%, respectively, at 15 degrees C. The diameter (mm) of the infection sites was reduced 91 and 96%, respectively, when compared to slices treated with F. sambucinum alone. Studies with Fusarium-infected whole potato tubers also showed significant reduction in dry rot formation following treatment with the bacterial isolates or the fungicide thiabendazole. When applied simultaneously with the pathogen, S. grimesii 4-9 and S. plymuthica 5-6 suppressed development of Fusarium dry rot by 60 and 77%, respectively, at 15 degrees C and by 63 and 84%, respectively, at 25 degrees C compared to tubers inoculated with the pathogen alone. Thiabendazole suppressed development of Fusarium dry rot by 66 and 81% at 15 and 25 degrees C, respectively, compared to tubers inoculated with the pathogen alone. These studies demonstrate the potential of soil bacteria as biofungicides for managing post-harvest crop diseases. Due to the potential risks to human health associated with S. grimesii 4-9, S. plymuthica 5-6 is recommended for further study for biofungicide development.