Egg-specific IgE and basophil activation but not egg-specific T-cell counts correlate with phenotypes of clinical egg allergy.
Egg-specific IgE and basophil activation but not egg-specific T-cell counts correlate with phenotypes of clinical egg allergy.
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DOI：
10.1016/j.jaci.2018.01.044
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Sampson HA
中科院分区：
文献类型：
--
作者：
Berin MC;Grishin A;Masilamani M;Leung DYM;Sicherer SH;Jones SM;Burks AW;Henning AK;Dawson P;Grabowska J;Agashe C;Davidson WF;Wood RA;Sampson HA
鸡蛋过敏是一种表型异质性。对鸡蛋过敏的一部分人可以耐受广泛加热的鸡蛋。在他们的饮食中加入烤蛋(BE)可以加速鸡蛋过敏的解决。相反，BE的反应性与持续性疾病有关。这种临床异质性的免疫基础尚不清楚。目的：研究反应性或耐受性BE患儿的虫卵特异性抗体、嗜碱性粒细胞和T细胞反应。所有参与者都接受了双盲、安慰剂对照的BE挑战，那些耐受BE的人接受了未加热的蛋清蛋白的挑战，以确认临床上的鸡蛋反应。实验室研究包括血清抗体、嗜碱性粒细胞活化试验和基于CD154的卵子反应性T细胞的流式细胞术检测。在129名被研究的儿童中，BE反应性参与者的卵子、卵清蛋白和卵粘蛋白特异性IgE水平显著较高，鸡蛋特异性IgG4/IgE比率较低，对鸡蛋的反应增加了嗜碱性粒细胞的激活。在所有参与者中，基于CD154的图谱显示鸡蛋反应性T细胞产生IL-4和IL-13，但很少IL-10或干扰素-γ，以及鸡蛋反应性Foxp3+CD25+CD127低Tregs的存在。鸡蛋反应性T细胞表达CCR4、CCR6和CXCR5，表明有能力归巢到皮肤、粘膜和B细胞毛囊。然而，在那些有耐受性或反应性的人中，鸡蛋反应T细胞的频率和表型都没有不同。在具有反应性和耐受性的患者中，虫卵特异性抗体和嗜碱性细胞反应较高，但T细胞反应不明显。在这个队列中，虫卵特异性抗体和T细胞反应是高度不同的。这种免疫异质性的临床意义需要进行纵向研究。在一项对129名接受烘焙和未加热鸡蛋挑战的儿童的研究中，鸡蛋特异性IgE和嗜碱性粒细胞活性显著降低，但鸡蛋特异性Th2细胞或Tregs的差异与BE的耐受性无关。
Egg allergy is phenotypically heterogeneous. A subset of egg allergic individuals can tolerate egg in an extensively heated form. Inclusion of baked-egg (BE) into their diet accelerates resolution of egg allergy. Conversely, BE reactivity is associated with persistent disease. The immune basis of this clinical heterogeneity is unknown. To study egg-specific antibody, basophil, and T cell responses in children with reactivity or tolerance to BE. All participants underwent double-blind placebo-controlled challenges to BE, and those who tolerated BE were challenged to unheated egg white protein to confirm clinical egg reactivity. Laboratory studies included serum antibodies, basophil activation tests, and CD154-based detection of egg responsive T cells by flow cytometry. Of the 129 children studied, BE reactive participants had significantly higher levels of egg, ovalbumin, and ovomucoid-specific IgE, lower ratios of egg-specific IgG4/IgE, and increased basophil activation in response to egg. Among all participants, CD154-based profiling revealed egg-responsive T cells producing IL-4 and IL-13, but little IL-10 or IFN-γ, as well as presence of egg-responsive Foxp3+CD25+CD127low Tregs. Egg-responsive T cells expressed CCR4, CCR6, and CXCR5, indicating capacity for homing to skin, mucosa, and B cell follicles. However, neither the frequency nor phenotype of egg-responsive T cells were different in those with tolerance or reactivity to BE. Egg-specific antibody and basophil responses, but not T cell responses, are higher in those with reactivity versus tolerance to BE. The egg-specific antibody and T cell responses were highly heterogeneous in this cohort. The clinical implications of this immune heterogeneity will need to be studied longitudinally. In a study of 129 children challenged to baked and unheated forms of egg, significantly lower egg-specific IgE and basophil activation, but no differences in egg-specific Th2 cells or Tregs, were associated with tolerance to BE.
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DOI：
10.2500/ar.2015.6.0135
发表时间：
2015-01
期刊：
Allergy & rhinology (Providence, R.I.)
影响因子：
--
作者：
Bartnikas LM;Sheehan WJ;Tuttle KL;Petty CR;Schneider LC;Phipatanakul W
通讯作者：
Phipatanakul W
影响因子：
14.2
作者：
Chiang, David;Chen, Xintong;Berin, M. Cecilia
通讯作者：
Berin, M. Cecilia
影响因子：
4.4
作者：
Bloom, Katherine A.;Huang, Faith R.;Nowak-Wegrzyn, Anna
通讯作者：
Nowak-Wegrzyn, Anna
影响因子：
14.2
作者：
Shreffler, Wayne G.;Wanich, Niya;Sampson, Hugh A.
通讯作者：
Sampson, Hugh A.
DOI：
10.1016/j.jaip.2013.04.002
发表时间：
2013-07-01
影响因子：
9.4
作者：
Bartnikas, Lisa M.;Sheehan, William J.;Phipatanakul, Wanda
通讯作者：
Phipatanakul, Wanda