PathGrid: a service-orientated architecture for microscopy image analysis
PathGrid: a service-orientated architecture for microscopy image analysis
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DOI：
10.1098/rsta.2010.0158
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发表时间：
2010-08-28
期刊：
影响因子：
5
通讯作者：
Rixon, G. T.
中科院分区：
文献类型：
--
作者：
Walton, N. A.;Brenton, J. D.;Rixon, G. T.
本文描述了“PathGrid”-一个分析和数据集成系统，最初是为了满足医学显微镜成像数据分析的需求而开发的。给出了当前系统的概述，描述了用于开发数据处理基础设施和分析算法开发的技术。注意到在为天文学领域设计的系统背景下创建的软件的使用，特别是用于数据发现、访问和工作流管理的天文学虚拟天文台运动的基础设施，以及用于分析高通量天文学成像调查的天文图像分析软件。本文指出了天文学领域技术的适用性。描述了测试平台基础设施的部署，强调了它的速度、易用性和支持。分析技术的有效性通过本文描述的初步研究得到了证实，该研究应用于大量免疫组织化学显微镜数据样本，部分用于评估乳腺癌的雌激素受体状态。分析表明，使用PathGrid自动评分的特异性和敏感性值在通过“金标准”手动病理学家评分获得的误差范围内。
This paper describes 'PathGrid'-an analysis and data integration system, developed initially to meet the demands in the analysis of medical microscopy imaging data. An overview of the current system is given, describing the techniques used in developing the data handling infrastructure and the analysis algorithm development. The use of software created in the context of systems designed for the astronomy domain is noted, specifically infrastructure from the astronomy virtual observatory movement for data discovery, access and workflow management, and astronomical image analysis software adapted for the analysis of high-throughput astronomy imaging surveys. This paper notes the applicability of the techniques from the astronomy domain. The testbed infrastructure deployment is described, emphasizing its speed and ease of use and support. The validity of the analysis techniques is confirmed through the pilot study described here-with the application to a large sample of immunohistochemistry microscopy data obtained in part for assessing the oestrogen receptor status of breast cancers. The analysis showed that the specificity and sensitivity values for the automatic scoring using PathGrid were within the errors of those obtained via a 'gold standard' manual pathologist scoring.