Macroscale spatial variation in chronic wound microbiota: a cross-sectional study.
Macroscale spatial variation in chronic wound microbiota: a cross-sectional study.
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DOI：
10.1111/j.1524-475x.2010.00628.x
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发表时间：
2011-01
期刊：
影响因子：
--
通讯作者：
Zenilman JM
中科院分区：
文献类型：
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作者：
Price LB;Liu CM;Frankel YM;Melendez JH;Aziz M;Buchhagen J;Contente-Cuomo T;Engelthaler DM;Keim PS;Ravel J;Lazarus GS;Zenilman JM
样本部位的控制被认为是慢性伤口微生物学研究的一个重要方面;然而，伤口微生物群的宏观空间变化尚未得到很好的表征。在13个慢性伤口的前缘、相对前缘和/或中心处共收集了31个刮匙样本。使用基于16 S rRNA基因的焦磷酸测序、热图显示、分层聚类、非度量多维标度和排列多变量方差分析的组合表征细菌群落组成。在13个伤口中，共有58个细菌科和91个细菌属。虽然在伤口中观察到大量的宏观尺度空间变化，但个体伤口内不同部位的细菌群落比不同伤口中的细菌群落显著更相似（p = 0.001）。我们的研究结果支持流行的观点，即控制采样部位可以提高伤口微生物群研究的质量;然而，来自个体伤口内不同部位的细菌群落的显著相似性表明，不应仅基于此标准而忽视未能控制采样部位的研究。来自各个伤口表面上多个部位的复合样品可以提供伤口微生物群的最稳健表征。
Controlling for sample site is considered to be an important aspect of chronic wound microbiological investigations; yet, macro-scale spatial variation in wound microbiota has not been well characterized. A total of 31 curette samples were collected at the leading edge, opposing leading edge and/or center of 13 chronic wounds. Bacterial community composition was characterized using a combination of 16S rRNA gene based pyrosequencing; heat map display; hierarchical clustering; non-metric multidimensional scaling; and permutation multivariate analysis of variance. A total of 58 bacterial families and 91 bacterial genera were characterized among the 13 wounds. While substantial macro-scale spatial variation was observed among the wounds, bacterial communities at different sites within individual wounds were significantly more similar than those in different wounds (p = 0.001). Our results support the prevalent opinion that controlling for sample site may improve the quality of wound microbiota studies; however, the significant similarity in bacterial communities from different sites within individual wounds indicates that studies failing to control for sampling site should not be disregarded based solely on this criterion. A composite sample from multiple sites across the surface of individual wounds may provide the most robust characterization of wound microbiota.