The long form of CDK2 arises via alternative splicing and forms an active protein kinase with cyclins A and E
The long form of CDK2 arises via alternative splicing and forms an active protein kinase with cyclins A and E
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DOI：
10.1089/104454901750361479
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发表时间：
2001-07-01
影响因子：
3.1
通讯作者：
Hunt, T
中科院分区：
文献类型：
--
作者：
Ellenrieder, C;Bartosch, B;Hunt, T
我们重新研究了在许多啮齿动物细胞中表达的细胞周期蛋白依赖性激酶2（CDK2）的长型形式。我们表明，编码CDK2L的信使核糖核酸（mRNA）是通过可变剪接产生的，并且所编码的蛋白质能够与细胞周期蛋白A和E结合并被其激活。CDK2L与细胞周期蛋白A形成的复合物的比活性约为等效的CDK2 - 细胞周期蛋白A复合物的一半。此外，CDK2L - 细胞周期蛋白A受到p21（CIP1）抑制的程度以及所需p21（CIP1）的浓度与CDK2 - 细胞周期蛋白A相同。人源和鼠源CDK2基因中内含子V的核苷酸序列（CDK2L中编码48个氨基酸插入片段的序列就位于此处）在可变剪接外显子及其周边区域的位置上显示出高度的保守性。尽管如此，我们无法在人类细胞系中检测到CDK2L的显著表达，尽管在猴源的COS - 1细胞中有低水平的表达。
We have reinvestigated the long form of cyclin-dependent kinase (CDK)2 that is expressed in many rodent cells. We show that the mRNA encoding CDK2L arises by alternative splicing and that the encoded protein can bind to, and be activated by, cyclins A and E. The complex of CDK2L with cyclin A has about half the specific activity of the equivalent CDK2-cyclin A complex. Also, CDK2L-cyclin A is inhibited to the same extent and by the same concentrations of p21(CIP1) as CDK2-cyclin A. The nucleotide sequences of intron V in the human and murine CDK2 genes, where the sequences encoding the 48-residue insert in CDK2L are located, show very high conservation in the position of the alternatively spliced exon and its surroundings. Despite this, we were not able to detect significant expression of CDK2L in human cell lines, although a low level is expressed in COS-1 cells from monkeys.