Developmental toxicity evaluation of three hexabromocyclododecane diastereoisomers on zebrafish embryos
Developmental toxicity evaluation of three hexabromocyclododecane diastereoisomers on zebrafish embryos
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三种六溴环十二烷非对映异构体对斑马鱼胚胎的发育毒性评价
DOI：
10.1016/j.aquatox.2012.01.013
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发表时间：
2012-05-15
影响因子：
4.5
通讯作者：
Zhang, Xian
中科院分区：
文献类型：
--
作者：
Du, Miaomiao;Zhang, Dandan;Zhang, Xian
六溴环十二烷非对映异构体的结构差异可能会导致物理化学、生物和毒理学特性的显着差异。为了全面评估六溴环十二烷（HBCD）的环境安全和健康风险，利用斑马鱼胚胎评估单个HBCD非对映异构体（α-HBCD、β-HBCD和γ-HBCD）的发育毒性。受精后 (hpf) 4 小时，斑马鱼胚胎暴露于不同浓度的 HBCD 非对映异构体（0、0.01、0.1 和 1.0 mg/l），直至 120 hpf。结果表明，接触六溴环十二烷可以以剂量依赖性和非对映选择性的方式影响斑马鱼胚胎/幼虫的发育。 0.01 γ-HBCD 的非对映异构体 α-、(β-和 γ-HBCD) 对斑马鱼胚胎的发育几乎没有影响，只是暴露于 0.01 mg/l γ-HBCD 会显着延迟孵化 (P < 0.05)。在 0.1 mg/l 时，α-HBCD 会导致幼鱼心率下降 (96 hpf) 并延迟孵化，而 β- 和 γ-HBCD 都会导致斑马鱼胚胎发育延迟。此外，在 0.1 mg/l γ-HBCD 暴露组中，α-、β-和 γ-HBCD 显着影响所有监测终点 (P < 0.05)，死亡率和畸形率显着增加 (P < 0.05)。结果表明，HBCD 非对映异构体可通过 ROS 形成诱导细胞凋亡，从而对斑马鱼胚胎产生发育毒性。总体结果显示，HBCD 非对映异构体对斑马鱼的发育毒性顺序为 γ-HBCD > β-HBCD > α-HBCD，版权所有 (C) 2012。 Elsevier B.V. 保留所有权利。
Structural dissimilarities of hexabromocyclododecane diastereoisomers could raise substantial differences in physicochemical, biological and toxicological properties. In order to fully assess the environmental safety and health risk of hexabromocyclododecanes (HBCDs), zebrafish embryos were used to evaluate the developmental toxicity of individual HBCD diastereoisomers (alpha-HBCD, beta-HBCD and gamma-HBCD). Four-hour post-fertilization (hpf) zebrafish embryos were exposed to different concentrations of HBCD diastereoisomers (0, 0.01, 0.1 and 1.0 mg/l) until 120 hpf. The results showed that exposure to HBCDs can affect the development of zebrafish embryos/larvae in a dose-dependent and diastereoselective manner. The diastereoisomers alpha-, (beta- and gamma-HBCD at 0.01 gamma-HBCD had little effect on the development of zebrafish embryos except that exposure to 0.01 mg/l gamma-HBCD significantly delayed hatching (P < 0.05). At 0.1 mg/l, alpha-HBCD resulted in depressed heart rate of larvae (96 hpf) and delayed hatching, whereas beta- and gamma-HBCD both caused significant hatching delay and growth inhibition (P < 0.05). In addition, a remarkable and significant increase in mortality and malformation rate was noted at 0.1 mg/l gamma-HBCD exposure groups (P < 0.05). At 1.0 mg/l, alpha-, beta- and gamma-HBCD significantly affected all of the endpoints monitored (P < 0.05). Additionally, HBCD diastereoisomers could induce the generation of reactive oxygen species (ROS) and the activities of caspase-3 and caspase-9 in a dose-dependent manner. The results indicated that HBCD diastereoisomers could cause developmental toxicity to zebrafish embryos through inducing apoptosis by ROS formation. The overall results showed a good agreement confirming that the order of developmental toxicity of HBCD diastereoisomers in zebrafish is gamma-HBCD > beta-HBCD > alpha-HBCD. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.