Disaccharide analysis of skin glycosaminoglycans in atrophoderma of Pasini and Pierini
Disaccharide analysis of skin glycosaminoglycans in atrophoderma of Pasini and Pierini
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DOI：
10.1046/j.1365-2230.2000.00682.x
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发表时间：
2000-07-01
影响因子：
4.1
通讯作者：
Ishikawa, O
中科院分区：
文献类型：
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作者：
Yokoyama, Y;Akimoto, S;Ishikawa, O
关于 Pasini 和 Pierini 萎缩皮病 (APP) 是一个疾病分类实体还是原发性萎缩性硬斑病，存在不同意见。在本研究中，我们使用高效液相色谱法分析了两名 APP 患者的皮肤糖胺聚糖 (GAG) 含量，并将结果与典型萎缩性硬斑病患者的结果进行了比较。每个患者的病灶周围未受累皮肤用作对照。在萎缩期硬斑病中，每次皮肤穿孔活检的二糖总量和每毫克干重的 Delta Di-4S(DS)（硫酸皮肤素的主要二糖单位）含量均有所增加。这些变化与硬化期形态一致。相反，APP中每次皮肤穿刺活检的二糖总量减少，每毫克干重的Delta Di-4S(DS)量减少或不变。我们的结果表明，APP 中的 GAG 代谢可能是独特的，并且与硬质藻中的 GAG 代谢有很大不同。
There are divergent opinions as to whether atrophoderma of Pasini and Pierini (APP) is a nosologic entity or a primary atrophic morphoea. In this study, we used high performance liquid chromatography to analyse the skin disaccharide contents of glycosaminoglycan (GAG) in two patients with APP and compared the results with those from a typical atrophic morphoea patient. Perilesional uninvolved skin was used as a control in each patient. In the atrophic phase morphoea, both the total amount of disaccharide per skin punch-biopsy and the amount of Delta Di-4S(DS) - the main disaccharide unit of dermatan sulphate - per mg dry weight were increased. These changes were consistent with sclerotic phase morphoea. In contrast, the total amount of disaccharide per skin punch-biopsy was decreased and the amount of Delta Di-4S(DS) per mg dry weight was decreased or unchanged in APP. Our results suggest that GAG metabolism in APP may be unique and quite different from that in morphoea.