Functional characterization of a novel jasmonate ZIM-domain interactor (NINJA) from upland cotton (Gossypium hirsutum)
Functional characterization of a novel jasmonate ZIM-domain interactor (NINJA) from upland cotton (Gossypium hirsutum)
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来自陆地棉（Gossypium hirsutum）的新型茉莉酸 ZIM 结构域相互作用子（NINJA）的功能表征
DOI：
10.1016/j.plaphy.2017.01.005
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发表时间：
2017
影响因子：
6.5
通讯作者：
Wu Jia-He
中科院分区：
文献类型：
--
作者：
Wang Le;Wu Shu-Ming;Zhu Yue;Fan Qiang;Zhang Zhen-Nan;Hu Guang;Peng Qing-Zhong;Wu Jia-He
茉莉酸(JA)信号通路在植物发育和抵御生物和非生物胁迫中起着重要作用。我们分离到一个棉花NINJA基因，命名为GhNINJA，它含有一个1305bp的开放阅读框。GhNINJA1基因编码434个氨基酸。实时荧光定量聚合酶链式反应分析表明，GhNINJA1基因主要在根中表达，其表达水平受黄萎病菌侵染的影响较大。通过病毒诱导的基因沉默技术，我们培育了GhNINJA沉默的棉花植株，显著降低了目的基因的表达，平均表达水平为对照的6%。与对照相比，沉默植株的再生侧根生长受到很大程度的抑制。显微镜分析表明，GhNINJA沉默植株的根分化区细胞长度明显短于对照。此外，与对照相比，沉默的植株对白粉病表现出更高的耐性，这与防御标记基因PDF1.2和PR4的表达增加有关。这些数据表明，GhNINJA基因的敲除抑制了根的生长，提高了对大丽花的耐受性。因此，GhNINJA1基因可作为棉花新品种选育的候选基因，提高棉花产量和抗病能力。
The jasmonic acid (JA) signalling pathway plays roles in plant development and defence against biotic and abiotic stresses. We isolated a cottonNINJA(novelinteractor ofJAZIM-domain) gene, designatedGhNINJA, which contains a 1305 bp open read frame. TheGhNINJAgene encodes a 434 amino acid peptide. According to quantitative real-time PCR analysis,GhNINJAis preferentially expressed in roots, and its expression level is greatly induced by Verticillium dahliae infection. Through a virus-induced gene silencing technique, we developedGhNINJA-silencedcotton plants, which had significantly decreased expression of the target gene with an average expression of 6% of the control. The regenerating lateral root growth of silenced plants was largely inhibited compared to the control. Analysis by microscopy demonstrated that the cell length of the root differentiation zone inGhNINJA-silenced plants is significantly shorter than those of the control. Moreover, the silenced plants exhibited higher tolerance toV. dahliaeinfection compared to the control, which was linked to the increased expression of the defence marker genesPDF1.2andPR4. Together, these data indicated that knockdown of GhNINJA represses the root growth and enhances the tolerance toV. dahliae. Therefore,GhNINJAgene can be used as a candidate gene to breed the new cultivars for improving cotton yield and disease resistance.