Essential oils of Mentha viridis rich phenolic compounds show important antioxidant, antidiabetic, dermatoprotective, antidermatophyte and antibacterial properties
Essential oils of Mentha viridis rich phenolic compounds show important antioxidant, antidiabetic, dermatoprotective, antidermatophyte and antibacterial properties
复制标题
DOI：
10.1016/j.bcab.2019.101471
复制
发表时间：
2020-01-01
影响因子：
4
通讯作者：
Bakri, Youssef
中科院分区：
文献类型：
--
作者：
Bouyahya, Abdelhakim;Lagrouh, Fatima;Bakri, Youssef
本研究旨在研究绿色薄荷精油(MVEO)的体外抗氧化、抗糖尿病、皮肤保护、抗皮肤真菌和抗菌作用。用气相色谱-质谱法测定了MVEO的化学成分。用DPPH、FRAP和ABTS三种互补方法评价其抗氧化活性。通过抑制α-淀粉酶和α-葡萄糖苷酶活性评价其体外抗糖尿病作用。通过酪氨酸酶和弹性蛋白酶的抑制作用来揭示MVEO的皮肤保护作用。采用琼脂扩散法和微量滴定法对5株菌株进行了抑菌活性测定。MVEO的化学成分分析表明，主要成分为香芹酮(37.26)、1.8-桉叶素(11.82)和松油烯-4-醇(08.72%)。DPPH法、FRAP法和ABTS法的IC50值分别为80.45+/-1.86mU/m L、101.78+/-3.14mU/m L和139.59+/-3.12m U/m L，具有显著的抗氧化作用。体外对氢化物降解相关酶的抑制实验表明，亚乙醇胺对α-淀粉酶(IC_(50)=101.72+/-1.86mU/m L)和α-葡萄糖苷酶(IC_(50)=86.93+/-2.43mU/m L)有较强的抑制作用。对酪氨酸酶(IC_(50)=55.13+/-1.01mU/mL)和弹性蛋白酶(IC_(50)=114.24+/-1.22mU/L)也有明显的抑制作用。对三种皮肤真菌(黑粉菌、黑粉菌和紫花粉菌)的抗真菌活性显示出重要的抑制作用。其抗菌活性对供试菌株有重要的杀菌作用，特别是对金黄色葡萄球菌和单核细胞增多性李斯特菌(MIC=MBC=0.25%)。这项工作的结果表明，MVEO的挥发性成分可以构成一种新的来源，具有抗氧化、抗糖尿病、皮肤保护、抗皮肤真菌和抗菌等特性。然而，关于MVEO主要化合物的分离和生物学特性的研究还需要进一步的研究。
The aim of this study was the investigation of the in vitro antioxidant, antidiabetic, dermatoprotective, anti-dermatophyte, and antibacterial properties of Mentha viridis essential oil (MVEO). Chemical composition of MVEO was determined using GC-MS analysis. Antioxidant activity was estimated by three complementary methods: DPPH, FRAP and ABTS. The in vitro antidiabetic effect was evaluated by the inhibition of alpha-Amylase and alpha-Glucosidase. Inhibitory effects of tyrosinase and elastase were used to reveal the dermatoprotective property of MVEO. Antibacterial activity was tested against five strains using agar well diffusion assay and microtitration method. The chemical composition determination of MVEO revealed the presence of carvone (37.26), 1.8-Cineole (11.82), and Terpinen-4-ol (08.72%) as the main compounds. MVEO presented remarkable antioxidant effects by IC50 values of 80.45 +/- 1.86 mu g/mL, 101.78 +/- 3.14 mu g/mL, and 139.59 +/- 3.12 mu g/mL obtained by DPPH, FRAP and ABTS tests respectively. In vitro inhibition of enzymes implicated in hydrocarbures degradation showed that MVEO exhibited important inhibitory effects against alpha-Amylase (IC50 = 101.72 +/- 1.86 mu g/mL) and alpha-Glucosidase (IC50 = 86.93 +/- 2.43 mu g/mL). Moreover, MVEO demonstrated remarkable inhibition of tyrosinase (IC50 = 55.13 +/- 1.01 mu g/mL) and elastase (IC50 = 114.24 +/- 1.22 mu g/mL). The antifungal activity showed important inhibitions of three dermatophyte species (T. mentagrophytes, T. tonsurans and T. violaceum). The antibacterial activity showed an important bactericidal action against tested strains, especially, Staphylococcus aureus and Listeria monocytogenes (MIC = MBC = 0.25%). The findings of this work showed that volatile components of MVEO could constitute a new source for antioxidant, antidiabetic, dermato protective, antidermatophyte and antibacterial properties. However, further investigations regarding the isolation of MVEO main compounds and investigations of their biological properties are required.