PUM1 promotes ovarian cancer proliferation, migration and invasion
PUM1 promotes ovarian cancer proliferation, migration and invasion
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PUM1促进卵巢癌增殖、迁移和侵袭
DOI：
10.1016/j.bbrc.2018.02.078
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发表时间：
2018-02-26
影响因子：
3.1
通讯作者：
Zong, Zhi-Hong
中科院分区：
文献类型：
--
作者：
Guan, Xue;Chen, Shuo;Zong, Zhi-Hong
背景：Pumilio RNA结合家族成员1（Pumilio RNA binding family member 1）基因的异常表达与染色体突变和肿瘤发生密切相关。然而，目前还没有关于卵巢癌组织中BMP 1的表达和功能的报道。方法：采用免疫组织化学方法检测卵巢癌组织和正常卵巢组织中BMP 1的表达，并与正常卵巢组织进行比较，探讨BMP 1在卵巢癌发生发展中的作用。在卵巢癌细胞系A2780中，使用小干扰RNA沉默了p53 1基因。MTT法、平板集落形成法和EdU（5-乙炔基-2 '-脱氧尿苷）法检测细胞生长情况，流式细胞仪检测细胞凋亡情况。进行伤口愈合和Transwell测定以确定细胞迁移和侵袭。结果：免疫组化结果显示卵巢癌组织中BMP 1的表达水平明显高于正常卵巢组织。细胞增殖、迁移和侵袭能力明显下降，而细胞凋亡增加。Western blotting结果显示，下调BCL 1表达可降低STAT 3、BCL 2、MMP 2和VEGFA的表达水平。结论：BCL 1可能通过上述分子促进卵巢癌的发生发展。(C)2018爱思唯尔公司All rights reserved.
Background: Abnormal expression of the PUM1 gene (Pumilio RNA binding family member 1) is closely related to chromosomal mutations and carcinogenesis. However, there is no report about expression or function of PUM1 in ovarian cancer. The present study explored the role of PUM1 in the development and progression of ovarian cancer.Methods: Immunohistochemistry was used to detect the expression of PUM1 in normal ovarian tissues and ovarian cancer tissues. The PUM1 gene was silenced using small interfering RNAs in ovarian cancer cell line A2780. MTT, plate colony formation and EdU (5-ethynyl-2'-deoxyuridine) assays were used to detect cell growth, and cell apoptosis was detected by flow cytometry. Wound-healing and Transwell assays were performed to determine cell migration and invasion. Western blotting was used to detect the levels of cancer-related proteins.Results: Immunohistochemistry showed that the level of PUM1 in ovarian cancer tissues was higher than that in normal tissues. The cell proliferation, migration, and invasion ability decreased significantly, while cell apoptosis increased after silencing the PUM1 gene. Moreover, western blotting showed that downregulation of PUM1 decreased the levels of STAT3, BCL2, MMP2, and VEGFA.Conclusions: Thus, PUM1 promotes the development and progression of ovarian cancer, which may occur via the above-mentioned molecules. (C) 2018 Elsevier Inc. All rights reserved.