Genetic analysis of the role of G protein-coupled receptor signaling in electrotaxis.
Genetic analysis of the role of G protein-coupled receptor signaling in electrotaxis.
复制标题
G蛋白偶联受体信号在电触及术中的作用的遗传分析。
DOI：
10.1083/jcb.200112070
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发表时间：
2002-06-10
影响因子：
7.8
通讯作者：
Devreotes, Peter N
中科院分区：
文献类型：
--
作者：
Zhao, Min;Jin, Tian;McCaig, Colin D;Forrester, John V;Devreotes, Peter N
Cells display chemotaxis and electrotaxis by migrating directionally in gradients of specific chemicals or electrical potential. Chemotaxis in Dictyostelium discoideum is mediated by G protein–coupled receptors. The unique Gβ is essential for all chemotactic responses, although different chemoattractants use different receptors and Gα subunits. Dictyostelium amoebae show striking electrotaxis in an applied direct current electric field. Perhaps electrotaxis and chemotaxis share similar signaling mechanisms? Null mutation of Gβ and cAMP receptor 1 and Gα2 did not abolish electrotaxis, although Gβ-null mutations showed suppressed electrotaxis. By contrast, G protein signaling plays an essential role in chemotaxis. G protein–coupled receptor signaling was monitored with PHcrac–green fluorescent protein, which translocates to inositol phospholipids at the leading edge of cells during chemotaxis. There was no intracellular gradient of this protein during electrotaxis. However, F-actin was polymerized at the leading edge of cells during electrotaxis. We conclude that reception and transduction of the electrotaxis signal are largely independent of G protein–coupled receptor signaling and that the pathways driving chemotaxis and electrotaxis intersect downstream of heterotrimeric G proteins to invoke cytoskeletal elements.
