A root penetration model of Arabidopsis thaliana in phytagel medium with different strength
A root penetration model of Arabidopsis thaliana in phytagel medium with different strength
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不同强度植胶培养基中拟南芥根系穿透模型
DOI：
10.1007/s10265-017-0926-4
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发表时间：
2017-09-01
影响因子：
2.8
通讯作者：
Zhou, Yong
中科院分区：
文献类型：
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作者：
Yan, Jie;Wang, Bochu;Zhou, Yong
Phytagel 介质被评估为机械阻碍拟南芥根部的系统。研究 Phytagel 的机械特性并探索根部对机械刺激的反应可以促进植物培养和植物发育。通过单轴压缩试验测试0.5-2.0% phytagel 介质的断裂强度。设置不同的植物凝胶浓度来改变生长培养基中层的强度。根长、直线度和中等强度之间观察到负相关。当根伸长穿过松软的上层（0.6%）时，穿透率随着下层强度（0.6-1.2%）的增加而降低，浓度≥1.2%时所有根均不能穿透下层。根系可从坚硬的上层(0.6-1.2%)向柔软的下层(0.6%)生长，穿透率降低。当根系在松软的下层生长时，与上层强度相关的生长速率达到峰值。通过在软硬层之间插入中等层来种植植物，提高了根系对1.2%下层的渗透能力，且0.8%中等介质中根部的渗透率显着高于1.0%中的根系渗透率。结论是，所研究的 Phytagel 系统适合研究机械阻抗对拟南芥根伸长的影响。中等强度对根系穿透能力影响显着，驯化可以提高根系穿透能力。
Phytagel media were evaluated as systems to mechanically impede roots of A. thaliana. Studying mechanical properties of Phytagel and exploring the root response to mechanical stimulation can facilitate plant culture and plant development. Breaking strengths of 0.5-2.0% phytagel media were tested by uniaxial compression test. Different phytagel concentrations were set to alter the strength of layers in growth medium. Negative correlations were observed between root length, straightness and medium strength. When roots elongated through soft upper-layer (0.6%), penetration ratio decreased with the increase of lower-layer strength (0.6-1.2%) and all roots couldn't penetrate into lower-layer with concentration ≥1.2%. Roots could grow into soft lower-layer (0.6%) from hard upper-layer (0.6-1.2%), with decreased penetration ratio. When roots grew in soft lower-layer, the growth rate linked with upper-layer strength increased to peak. Roots penetration capability into 1.2% lower-layer was improved by growing plants through moderate layer inserted between soft and hard layer, and roots in 0.8% moderate medium have a significant higher penetration ratio than that in 1.0%. It was concluded that the Phytagel systems studied were suitable for studying the effect of mechanical impedance on the elongation of A. thaliana roots. The medium strength affected root penetration significantly and acclimation can improve root penetration capability.