Interactions between Radiation and Benzo(a)pyrene in an in vitro Model for Malignant Transformation
Interactions between Radiation and Benzo(a)pyrene in an in vitro Model for Malignant Transformation
复制标题
体外恶性转化模型中辐射与苯并(a)芘的相互作用
DOI：
10.1007/978-3-642-61939-7_48
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
J. Little
中科院分区：
文献类型：
--
作者：
Margaret Terzaghi;J. Little
BaP和X射线照射C_3H小鼠胚胎细胞系（10 T-1/2），诱发小鼠恶性转化。当两种药物顺序给药时，发现了协同效应;最大协同作用发生在治疗间隔15至20小时时。每存活细胞的存活率和转化频率都通过在辐射后4小时维持细胞在生长抑制的条件下而增强，这表明修复过程可能参与转化过程。这种体外转化系统，特别是涉及平台期文化，似乎是有价值的辅助化学致癌物和辐射之间的相互作用，可能会发生在肺中的关键细胞群的研究。
Malignant transformation, confirmed by reinjection of transformed cells into syngeneic mice, has been induced by both BaP and X-irradiation in a C3H mouse embryo cell line (10T-1/2). A synergistic effect was found when both agents were given sequentially; maximum synergism occurred when 15 to 20 hours elapsed between treatments. Both survival and transformation frequency per surviving cell were enhanced by maintaining cells in conditions of growth inhibition for 4 hours after radiation, suggesting that repair processes may be involved in the transformation process. Such an in vitro transformation system, particularly involving plateau phase cultures, appears to be valuable adjunct to the study of interactions between chemical carcinogens and radiation that may occur in critical cell populations in the lung.