IMMUNOHISTOCHEMICAL DEMONSTRATION OF POLY(ADENOSINE DIPHOSPHATE-RIBOSE) SYNTHETASE IN BOVINE-TISSUES
IMMUNOHISTOCHEMICAL DEMONSTRATION OF POLY(ADENOSINE DIPHOSPHATE-RIBOSE) SYNTHETASE IN BOVINE-TISSUES
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DOI：
10.1177/31.11.6311893
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
UEDA, K
中科院分区：
文献类型：
--
作者：
IKAI, K;UEDA, K
使用间接免疫荧光技术和针对从小牛胸腺纯化的酶的特异性抗体，研究了聚（ADP-核糖）合成酶的细胞间和细胞内定位。在各种牛组织中，包括肝脏、心脏、胰腺、甲状腺、脾脏、肾上腺和骨骼肌，聚（ADP-核糖）合成酶的特异性免疫荧光仅位于细胞核中。用纯化的小牛胸腺聚（ADP-核糖）合成酶预吸收抗体可抑制免疫染色。在大多数细胞核中，核免疫荧光似乎在边缘区域比在中心区域更突出。这种染色模式与天然存在的聚（ADP-核糖）的染色模式相似。在牛外周血中，在淋巴细胞核中检测到聚（ADP-核糖）合成酶的免疫荧光，但在粒细胞中未检测到，这与在从粒细胞分离的细胞核中几乎检测不到聚（ADP-核糖）合成酶的酶活性的发现一致。
The inter- and intracellular localization of poly(ADP-ribose) synthetase was investigated using an indirect immunofluorescence technique and a specific antibody against the enzyme purified from calf thymus. In various bovine tissues, including liver, heart, pancreas, thyroid, spleen, adrenal and skeletal muscle, the specific immunofluorescence of poly(ADP-ribose) synthetase was localized exclusively in the nucleus. Immunostaining was inhibited by preabsorption of the antibody with purifed calf thymus poly(ADP-ribose) synthetase. Nuclear immunofluorescence appeared to be more prominent in the marginal area than in the central region in most nuclei. This staining pattern is similar to that of naturally occurring poly(ADP-ribose). In bovine peripheral blood the immunofluorescence of poly(ADP-ribose) synthetase was detected in nuclei of lymphocytes, but not in granulocytes, in agreement with the finding that the enzymatic activity of poly(ADP-ribose) synthetase was barely detectable in nuclei isolated from granulocytes.